5-氟脲嘧啶诱发的小鼠睾丸形态学早期变化

目的:通过观察化疗药物5-氟脲嘧啶诱发小鼠睾丸组织形态学早期变化特点,提出一种简易实用的小鼠少精/弱精/无精模型制备方法,为相应生精机制研究和药物疗效观察提供方法学建议。方法:小鼠尾静脉一次性注射化疗药物5-氟脲嘧啶(250mg/kg),分别在注射前,以及注射后第3、7、11、14天取小鼠睾丸,制备石蜡切片,HE染色观察形态学变化。结果:小鼠睾丸内完整致密排列的生精小管管壁中各级精母细胞/精子细胞,在注射5-氟脲嘧啶之后第3、7、11天呈现进行性减少,管壁进行性变薄,在第11天达到最低点,并可见管壁明显肿胀和裂纹;第14天生精小管肿胀基本消失,但裂纹依然存在,可见精母细胞明显增多,但未见成熟精子。结论:小鼠尾静脉一次性注射5-氟脲嘧啶可能是一种简单、有效的化疗药物诱发生殖功能损伤动物模型制备方法。

Early Morphological Changes in the Mouse Testis Induced by 5-Fluorouracil

Objective:To propose a simple and practical method for preparing a mouse model of oligo-atheno-terato-spermia/azoosper-mia,and to offer a methodological suggestion for the studies on the related mechanism of spermatogenesis and evaluation of medication efficacy by observing the early changes of testis morphology after 5-fluorouracil treatment. Methods:Mice were injected with a single dose of 5-flourouracil at 250 mg/kg via the tail vein,and their testes were harvested and paraffin sections prepared before and 3,7,11 and 14 d after the injection to be observed for the morphological changes by hematoxylin and eosin staining. Results:The numbers of spermotocytes/spermatids were progressively reduced inside the testis seminiferous tubules of the mice at 3,7 and 11 d after the 5-fluorouracil injection,and the tubule walls became thinner,which reached the nadir at 11 d,with evident swelling and crazing of the seminiferous tubules.At 14 d,the swelling almost disappeared and spermotocytes became repopulated,while the flaws still existed in the seminiferous tubules and no mature sperm were seen. Conclusion:One-dose injection of 5-fluorouracil via the tail vein might be a simple and effective method for preparing the animal model of reproductive function damage induced by chemotherapeutic medication.