肝细胞癌组织中MMP-2的表达与侵袭转移的关系

目的:探讨人肝细胞性肝癌(hepatocellularcarcinoma,HCC)中基质金属蛋白酶2(matrixmetalloproteinase2,MMP2)的表达与其侵袭转移的关系。方法:用半定量逆转录PCR(RTPCR)方法检测癌、癌旁组织及正常组织中MMP2mRNA的表达;用免疫组织化学方法检测癌、癌旁组织及正常组织中MMP2蛋白质的表达。结果:MMP2在肝癌中的表达高于癌旁组织和正常组织,差异有统计学意义,P<0.05;癌旁组织和正常组织中MMP2的表达差异无统计学意义,P>0.05。癌组织中MMP2的表达与术后复发时间呈负相关,P=0.013,R2=0.842;与病理学分级呈正相关,P=0.000,R2=0.797。癌组织中MMP2的表达与肝外转移呈正相关,P=0.003,R2=0.834。癌旁组织中MMP2的表达与术后复发时间呈负相关,P=0.021,R2=0.877。结论:MMP2与肿瘤的分化程度、侵袭、转移能力和复发倾向相关。在临床中可望作为肿瘤分化、复发和转移的评估指标之一。

Relationship between MMP-2 and invasion and metastasis of hepatocellular carcinoma

OBJECTIVE:To investigate the association between MMP-2 expression of hepatocellular carcinoma (HCC) and its invasion and metastasis. METHODS: Semi-quantitative RT-PCR and immunohistochemical analysis technology were used to detect MMP-2 mRNA expression and MMP-2 protein expression respectively in HCC, non-HCC liver tissue samples and normal liver tissue samples. RESULTS: The level of MMP-2 expression in HCC was higher than that in adjacent non-HCC liver tissue and in normal liver tissue and the difference was statistically significant, P<0.05, and the difference of MMP-2 expression between adjacent non-HCC liver tissue and normal liver tissue had no statistical significance, P>0.05. MMP-2 expression in HCC had an inverse correlation with recurrence after surgical treatment, P=0.013, R2=0.842, and a positive correlation with pathological grade, P=0.000, R2=0.797. There was a positive correlation between MMP-2 expression in HCC and extrahepatic metastasis, P=0.003, R2=0.834. Significant inverse correlation was found between MMP-2 expression in adjacent non-HCC liver tissue and extrahepatic metastasis, P=0.021, R2=0.877. CONCLUSIONS: MMP-2 has an association with degree of tumor differentiation, capability of invasion and metastasis and tendency of recurrence. MMP-2 way be one of indexes of differentiation, recurrence and metastasis.