脂肪干细胞复合富血小板血浆对脂肪颗粒移植的影响

目的 探讨运用组织工程技术构建的组织工程脂肪对颗粒脂肪移植的影响。 方法 通过酶消化法获取脂肪干细胞(ASCs),采用两步离心法制备富血小板血浆(PRP),运用组织工程技术构建组织工程脂肪。根据移植物的组成不同分为ASCs+PRP+脂肪颗粒、PRP+脂肪颗粒、ASCs+脂肪颗粒及对照组(PBS+脂肪颗粒),分别移植入裸鼠皮下,并于移植后10 d、30 d、60 d及90 d取出移植物,进行大体观察及体积测量,镜下观察组织结构变化。 结果 90 d后ASCs+PRP+脂肪颗粒组移植物质地柔软、颜色淡黄,较其它组别更接近于正常的脂肪组织,且具有更多的存活体积(P<0.05)及更好的组织学形态。 结论 运用ASCs与PRP复合脂肪颗粒能够成功构建组织工程脂肪,是一种新的安全有效的修复颌面部及全身软组织缺损方法,具有较高的应用前景。

Increased Survival of Free Fat Grafts with Adipose-derived Stem Cells and Platelet-rich Plasma in Nude Mice

Objecitve To investigate the impact of combined adipose-derived stem cells (ASCs) and platelet-rich plasma (PRP) on the survival of transplanted fat. Methods The ASCs were isolated and cultured from fat tissues by enzyme digestion; and the PRP was prepared by two-step ultracentrifugation. The grafts of fat granules were divided into test groups (ASCs+PRP+fat granules, PRP+fat granules, ASCs+fat granules) and control groups (PBS+fat granules). The grafts were injected into the left and right dorsal subcutaneous areas of nude mice. General observations, volume measurements and microscope examinations were conducted 10 d, 30 d, 60 d and 90 d after transplantation. Results The grafts of the mice in the ASCs+PRP group showed soft structure, with light yellow color and closer to normal adipose tissue compared with those in other groups. Greater survival volumes (P<0.05) and better histology were also observed in the grafts of the mice in the ASCs+PRP group. Conclusion The fat grafts consisting of PRP and ASCs constitute an ideal transplant strategy, which could provide a valuable and needed tool in plastic and reconstructive surgery.