大鼠癫癎持续状态后海马神经元凋亡的研究

目的：通过美解眠诱导鼠癫癎持续状态后观察海马神经元的细胞凋亡现象。　材料和方法：给ＳＤ大鼠腹腔注射美解眠（２０ ｍ ｇ／ｋｇ），２４ ｈ 后在光镜和电镜下观察大鼠海马神经元的形态学改变，并以ＤＮＡ 末端标记法（ＴＵＮＥＬ）检测海马神经元的凋亡。　结果：ＳＤ大鼠注射美解眠后出现典型的癫癎发作，光镜和电镜下可观察到海马神经元有典型的细胞凋亡现象，包括细胞体皱缩、胞质浓缩、染色质凝聚成块状、有边集现象， 同时有少量凋亡小体形成。ＴＵＮＥＬ检测发现海马结构内可见ＴＵＮＥＬ染色阳性细胞，其分布不均匀，有区域性差异，以ＣＡ３ 区最为多见。致癎组海马ＣＡ３ 区ＴＵＮＥＬ阳性细胞数的平均百分率（７５．１４％ ）与对照组（９．４％ ）相比有极显著性差异（Ｐ＜ ０．０１）。　结论：癫癎发作可诱导大鼠海马神经元发生凋亡。

Study on hippocampal neuronal apoptosis in rats after status epilepticus

Objectives: To observe hippocampal neuronal apoptosis phenomenon in rats after status epilepticus(SE) induced by bemegride. Methods:SD rats were given bemegride( 20 mg/kg) intra abdominally,and after 24 hours the morphology of the hippocampal neurons was detected through light microscope and electron microscope.Meanwhile,In Situ End Labeling(TUNEL) was used to measure hippocampal neuronal apoptosis. Results:After being injected bemegride, the rats displayed characteristic seizure attacks.Under the light microscope and electron microscope,the hippocampal neurons displayed a characteristic pattern of structural changes in nucleus and cytoplasm, including blebbing of plasma membrane and nuclear disintegration, with some apoptotic bodies in formation.Positive TUNEL cells could be seen in the hippocampal structure,and the distribution was not the same,which was more in the hippocampal CA3 region .There was significant difference( P <0.01) in the average rate of hippocampal CA3 region positive TUNEL cells between the study group(75.14%) and the control(9.4%). Conclusions:SE can induce hippocampal neuronal apoptosis in rats.