中药黄蜀葵花化学成分的分离与鉴定(Ⅱ)

目的研究中药黄蜀葵(Abelmoschus manihot(L.)Medic.)花的化学成分,为进一步开发利用该植物资源提供依据。方法采用正相硅胶、反相ODS、Sephadex LH-20等柱色谱以及高效液相色谱等手段进行分离纯化,并通过理化性质与光谱分析鉴定化合物的结构。结果从黄蜀葵花体积分数为95%的乙醇提取物的大孔吸附树脂洗脱物中分离鉴定了9个以槲皮素为母核的黄酮类化合物,分别为槲皮素(quercetin,1)、金丝桃苷(hyperoside,2)、槲皮素-3-O-β-D-葡萄糖苷(quercetin-3-O-β-D-glucopyranoside,3)、槲皮素-3-O-β-D-6″-乙酰葡萄糖苷(quercetin-3-O-β-D-6″-acetylglucopyr-anoside,4)、槲皮素-3-O-刺槐糖苷(quercetin-3-O-robinoside,5)、槲皮素-3-O-芸香糖苷(quercetin-3-O-rutinoside,6)、槲皮素-3-O-β-D-木糖基-(1→2)-β-D-半乳糖苷(quercetin-3-O-β-D-xylopyranosyl(1→2)-β-D-galactopyranoside,7)、槲皮素-3′-O-β-D-葡萄糖苷(quercetin-3′-O-β-D-glucopyranoside,8)以及槲皮素-7-O-β-D-葡萄糖苷(quercetin-7-O-β-D-glucopyranoside,9)。结论化合物4、5、7和9为首次从秋葵属植物中分离得到。

Isolation and identification of chemical constituents from the flowers of Abelmoschus manihot (L.) Medic. (II)

Obejective To study the chemical constituents from the flowers of Abelmoschus manihot(L.)Medic. Methods The compounds were isolated by chromatography by using silica gel, ODS, Sephadex LH-20 and HPLC,and identified by the chemical and physical methods, especially spectral analysis. Results Nine flavonoids which have quercetin skeleton were isolated. The structures of them were identified as quercetin (1), hyperoside (2), quercetin-3-O-β-D-glucopyranoside (3), quercetin-3-O- β-D-6"-acetylglucopyranoside (4), quercetin-3-O-robinoside (5), quercetin-3-O-rutinoside (6), quercetin-3-O-β-D-xylopyranosyl-(1→2)-β-D-galactopyranoside (7), quercetin-3’-O-β-D-glucopyranoside (8) and quercetin-7-O-β-D-glucopyranoside (9), respectively. Conclusion Compounds 4, 5, 7 and 9 are obtained from Abelmoschus genus for the first time.