| 细菌内毒素检测试验方法的建立 |
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| 引用本文： | 王炜红,杨晶雪,陈微娜,曹原,杜丽,林玲,李俐,张馨心,梁琦,冯立民. 细菌内毒素检测试验方法的建立[J]. 中国消毒学杂志, 2011, 28(2) |
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| 作者姓名： | 王炜红 杨晶雪 陈微娜 曹原 杜丽 林玲 李俐 张馨心 梁琦 冯立民 |
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| 作者单位： | 黑龙江省疾病控制中心消毒监测管理所,哈尔滨,150030 |
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| 摘 要： | 目的建立医疗用水中细菌内毒素的检测方法。方法采用鲎试剂检测法,通过干扰试验和灵敏度复核试验方法,建立内毒素试验方法。结果经对72份透析液和透析用水进行检测,其中检出67份标本中细菌内毒素含量<2 EU/ml,检出5份标本中细菌内毒素含量>2 EU/ml。设定内毒素效价2.0～2.4 EU/ml(间隔0.1)范围内连续5组效价进行偏离实验,在30 d后仍然可检出内毒素存在。结论本研究建立的内毒素试验方法成立,试验结果支持,偏离试验解决了下基层检测医疗用水中内毒素的仪器设备及试验条件带来的限制。
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| 关 键 词： | 内毒素 鲎试验 灵敏度 偏离试验 |
THE ESTABLISHMENT OF EXPERIMENTAL METHOD FOR BACTERIAL ENDOTOXIN TEST |
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| WANG Wei-hong,YANG Jing-xue,CHEN Wei-na,CAO Yuan,DU Li,LIN Ling,LI Li,ZHANG Xin-xin,LIANG Qi,FENG Li-min. THE ESTABLISHMENT OF EXPERIMENTAL METHOD FOR BACTERIAL ENDOTOXIN TEST[J]. Chinese Journal of Disinfection, 2011, 28(2) |
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| Authors: | WANG Wei-hong YANG Jing-xue CHEN Wei-na CAO Yuan DU Li LIN Ling LI Li ZHANG Xin-xin LIANG Qi FENG Li-min |
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| Affiliation: | WANG Wei-hong,YANG Jing-xue,CHEN Wei-na,CAO Yuan,DU Li,LIN Ling,LI Li,ZHANG Xin-xin,LIANG Qi,FENG Li-min (Heilongjiang Provincial Centre for Disease Control and Prevention,Harbin 150030,China) |
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| Abstract: | Objective To establish the detection method of bacterial endotoxin in medical water. Methods Tachypleus amebocyte lysate detection was used through the interference test and sensitivity test to establish the endotoxin test method. Results Among 72 dialysis solution and dialysis water detected,bacterial endotoxin contents of 67 specimens were less than 2 EU/ml,and that of 5 specimens were greater than 2 EU/ml.The endotoxin potency was set from 2.0 EU/ml to 2.4 EU/ml(interval 0.1) for 5 groups within the devi... |
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| Keywords: | endotoxin limulus test sensitivity deviation test |
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