瘦素与p-mTOR 在大肠癌中的表达及临床意义

目的:探讨瘦素和p-mTOR 蛋白表达与大肠癌发生、发展及其临床病理特征之间的关系。方法:采用免疫组织化学SP 法检测瘦素、p-mTOR 在40 例正常大肠黏膜,40 例大肠腺瘤性息肉和108 例大肠癌中的表达。结果:瘦素在正常大肠黏膜组织、大肠腺瘤性息肉、大肠癌组织中的阳性表达率分别为10%(4/40),27.5%(11/40),71.3%(77/108),各组之间差异有统计学意义(P<0.05)。p-mTOR 在正常大肠黏膜组织、大肠腺瘤性息肉、大肠癌组织中的阳性表达率分别为2.5%(1/40),20%(8/40),61.1%(66/108),各组之间差异有统计学意义(P<0.05);瘦素与p-mTOR 的高表达与大肠癌的浸润深度、临床分期、淋巴结转移、远处转移、分化程度密切相关(P<0.05), 而与患者的年龄、性别和部位无关(P>0.05);大肠癌组织中瘦素与p-mTOR 的表达呈正相关(P<0.01)。结论:瘦素与p-mTOR 可能在大肠癌的发生、发展中起相互协同作用,二者可能作为判断大肠癌预后及指导临床治疗的指标。

Expression of leptin and p-mTOR and their clinicopathological significance in human colon carcinoma

Objective: To investigate the relationship between the expression of leptin,p-mTOR protein and the pathogenesis,development and clinicopathological features in colon carcinoma.Methods: The expression of leptin and p-mTOR protein was evaluated by immunohistochemical methodsin 40 normalcolonmucosas,40 colonadenomatouspolypsand 108casesofcoloncarcinomas.The relationship between the staining pattern and clinicopathogical features was examined.Results: The positive rates of detection of leptin in normal colon mucosa,adenomatous polyps and colon carcinomas were 10%(4/40),27.5%(11/40),and 71.3%(77/108),respectively;with significant differences among the three groups(P<0.05).The positive rates of p-mTOR protein in the normal colon mucosa,the adenomatous polyps,and the colon carcinomas were 2.5%(1/40),20%(8/40),and 61.1%(66/108),respectively;with significant differences among the three groups(P<0.05).The expression of leptin and p-mTOR proteins were related to invasive depth,TNM stages,lymph node metastasis,distant metastasis and tumor differentiation(P<0.05),but not to age,sex,or site(P>0.05).In colon carcinoma tissues,leptin expression was positively correlated with p-mTOR expression(P<0.01).Conclusion: Leptin and p-mTOR proteins may play important roles in the occurrence and development of colon carcinoma.The detection of leptin and p-mTOR may be helpful for evaluation of the prognosis of the patient with colon carcinoma.