| 雌激素通过miR-146a影响间充质干细胞的免疫调节功能 |
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| 引用本文： | 赵晓寅,赵叶琳,刘柳,侯亚义. 雌激素通过miR-146a影响间充质干细胞的免疫调节功能[J]. 现代免疫学, 2012, 0(3): 216-221 |
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| 作者姓名： | 赵晓寅 赵叶琳 刘柳 侯亚义 |
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| 作者单位： | 南京大学医学院免疫与生殖生物学实验室 |
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| 基金项目： | 国家自然科学基金项目(81072410);江苏省卫生厅兴卫工程基金(XK200709);江苏省第五批“六大人才高峰资助”项目资助 |
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| 摘 要： | 本研究用混合酶法分离培养人脐带间充质干细胞(MSC),检测在不同浓度17β-雌二醇(E2)作用下MSC中MicroR-NA的表达情况。进一步通过转染使MSC高表达miR-146a,并分析转染后MSC的表型、功能及相关因子的表达变化。结果显示,E2处理的MSC中IFN-β的表达没有发生明显变化,STAT1与IDO1的表达显著下调。同时,E2处理能够上调MSC对miR-146a的表达,降低其对miR-23b和miR-21的表达。miR-146a转染不影响MSC的表型,而显著降低STAT1和IDO的表达。提示E2可能通过影响MSC内miR-146a的表达改变其免疫调节功能。
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| 关 键 词： | 雌激素 间充质干细胞 miR-146a |
Estrogen can alter immunoregulatory function of MSC via miR-146a |
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| ZHAO Xiao-yin,ZHAO Ye-lin,LIU Liu,HOU Ya-yi. Estrogen can alter immunoregulatory function of MSC via miR-146a[J]. Current Immunology, 2012, 0(3): 216-221 |
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| Authors: | ZHAO Xiao-yin ZHAO Ye-lin LIU Liu HOU Ya-yi |
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| Affiliation: | (Immunology and Reproductive Biology Laboratory,Medical School of Nanjing University,Nanjing 210093,China) |
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| Abstract: | We used the mixed enzyme method for the isolation and culture of human umbilical cord mesenchymal stem cells(MSC),and investigated the expression of MicroRNA in MSC treated with 17β-Estradiol(E2) at different concentrations.Then the phenotypes,functions and the expression of related genes of MSC were analyzed after transfecting miR-146a into MSC.Our results showed that the expression of IFN-β did not change significantly in MSC treated with E2,while the expression of STAT1 and IDO1 were remarkably downregulated.The expression of miR-146a was remarkably enhanced while the expression of miR-23b and miR-21 were decreased.The transfection of miR-146a significantly inhibited the expression of STAT1 and IDO,without affecting the phenotypes of MSC.Therefore,we conclude that E2 may alter the immunoregulatory effects of MSC by modulating miR-146a expression. |
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| Keywords: | 17β-Estradiol mesenchymal stem cells miR-146a |
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