TFPI-2基因对Panc-1细胞凋亡的影响

目的]研究TFPI-2基因对胰腺癌细胞系Panc-1细胞增殖及凋亡的影响。方法]测定Panc-1-TFPI-2、Panc-1-V和Panc-1-P三组细胞的生长曲线．DNA片段化实验检测细胞凋亡，并用流式细胞仪分析三组细胞的细胞增殖、细胞周期情况。结果]Panc-1-TFPI-2细胞同Panc-1-V和Panc-1-P细胞相比，细胞生长缓慢，细胞生长受到抑制，被阻滞于G0～G1期；DNA片段化实验显示Panc-1-TFPI-2细胞呈现凋亡细胞特有的“梯状”条带．而Panc-1-V和Panc-1-P细胞无明显“梯状”条带：流式细胞仪分析显示早期Panc-1-TFPI-2细胞凋亡率（6．93％± 0．53％）较Panc-1-V（3．01％± 0．39％）和Panc-1-P细胞凋亡率（3．52％±0．41％）增加，有显著性差异（P〈0．05）。结论]TFPI-2能够抑制胰腺癌细胞生长、诱导胰腺癌细胞凋亡．可能具有抑制肿瘤细胞增殖的作用。

The Effect of TFPI-2 on Apoptosis of Pancreatic Carcinoma Cell Panc-1

Purpose] To investigate the effect of TFPI-2 gene on cell proliferation and apoptosis of human pancreatic cancer cell line Pane-1. Methods] The growth curve was detected in 3 groups, Panc-1-TFPI-2, Panc-1-V and Panc-1-P.DNA strand breaks was used to detect the apoptosis in the 3 groups. The change of cell cycle and apoptosis index were evaluated by flow cytometry. Resuhs] Compared with nontransfected cells （Pane-1-P, Pane-1-V）, the growth of Pane-1-TFPI-2 cell was slower and inhibited significantly.The transfeeted cells showed a significant increase in G0-G1 phase.The apoptosis of Panc-1-TFPI-2 cell was identified by DNA strand breaks and flow cytometry in comparison with the control group.The apoptosis index of the Panc-1-TFPI-2 cell （6.93%± 0.53%） was higher than that in Panc-1-V （3.01%± 0.39%） and Pane-1-P （3.52%± 0.41%）, with significant difference（P〈0.05）. Conclusion] The exogenous TFPI-2 gene can inhibit the growth and induce apoptosis of pancreatic cancer cell, which maybe inhibit the cancer cells proliferation.