附子和四逆汤表观药动学参数的测定

以毒性为指标测定附子及四逆汤的表观药动学参数。方法为:先测小鼠ipLD_(50),另取小鼠ip首次剂量后分为不同间隔时间组,分别通过LD_(50)补量法测定间隔不同时间后首次注入药物的体存量,以log体存量对用药后间隔时间作图得药物体存量的经时性变化曲线,并计算药动学参数。经判断附子和四逆汤在体内的经时性变化均符合二室模型。计算结果二药分布相的半衰期分别为1.15h及0.35h,消除相的半衰期分别为17.0h及5.8h。

Determination of apparent pharmacokinetic parameters of Radix Aconiti Lateralis Preparata and Si Ni Tang

Apparent pharmacokinetic parameters of Radix Aconiti Lateralis preparata and Si Ni Tang were determined by acute mortality of mice. l.The ip LD50 of mice was determined. 2.Mice received a dosage . (about LD10) were divided into 5-6 interval groups. For each group,ip LD50(t) was determined after appropriate interval.3.Residual of the first dosage after the interval of t =LD50-D50(t) . 4.on the basis of interval time and residual (log) relationship, a curve of dynamic change after initial dosage was drawn, and apparent pharmacokinetic parameters were calculated. The T1/2a of Radix Aconiti Lateralis preparata and Si Ni Tang were 1.15 h and 0.35 h, Ti/2B were 17.0 h and 5.8h respectively.