| 嘌呤受体P2Y_2在涎腺腺样囊性癌的表达及ATP对其细胞生长的抑制 |
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| 引用本文： | 柴松岭,高璐,向彬,张福胤. 嘌呤受体P2Y_2在涎腺腺样囊性癌的表达及ATP对其细胞生长的抑制[J]. 现代口腔医学杂志, 2010, 24(3): 194-197 |
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| 作者姓名： | 柴松岭 高璐 向彬 张福胤 |
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| 作者单位： | 1. 大连医科大学口腔医学院,116044
2. 大连大学医学院口腔系 |
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| 摘 要： | 目的研究嘌呤受体P2Y2在涎腺腺样囊性癌非高转移细胞株（SACC-83）和高转移细胞株（SACC-LM）的表达,及其激动剂ATP对二者细胞生长的抑制作用。方法采用免疫细胞化学法检测P2Y2受体在SACC-83和SACC-LM细胞株中的表达;采用MTT法检测不同浓度ATP（0.1mM/L、0.2mM/L、0.4mM/L、0.8mM/L和1.6mM/L）在不同作用时间点（24h、48h和72h）对SACC-83和SACC-LM细胞生长的抑制作用。结果 SACC-83和SACC-LM细胞中P2Y2均呈阳性表达,在细胞膜的染色较胞浆深,其表达在两细胞株中无明显差异。ATP对SACC-83和SACC-LM细胞的生长均有明显的抑制作用（P〈0.05）,并且对SACC-LM细胞的抑制作用强于对SACC-83细胞的抑制作用,其抑制作用与药物浓度和作用时间呈正相关。结论不同转移能力的涎腺腺样囊性癌细胞SACC-83和SACC-LM的胞膜和胞浆中均有嘌呤受体P2Y2表达,ATP对二者细胞生长有显著抑制作用。
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| 关 键 词： | 涎腺腺样囊性癌 嘌呤受体P2Y2 ATP |
Expression of P2Y_2 receptor in salivary adenoid cystic carcinoma and the inhibitive effect of ATP on cell proliferation |
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| CHAI Songling,GAO Lu,XIANG Bin,et al.. Expression of P2Y_2 receptor in salivary adenoid cystic carcinoma and the inhibitive effect of ATP on cell proliferation[J]. Journal of Modern Stomatology, 2010, 24(3): 194-197 |
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| Authors: | CHAI Songling GAO Lu XIANG Bin et al. |
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| Affiliation: | CHAI Songling,GAO Lu,XIANG Bin,et al. College of Stamatology,Dalian Medical University,Dalian 116044 |
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| Abstract: | Objective To investigate the expression of P2Y2 receptor in salivary adenoid cystic carcinoma cell lines (SACC-83 and SACC-LM) and the inhibitive effect of ATP on the cell growth.Methods The expression of P2Y2 receptor on SACC-83 and SACC-LM was measured by immunocytochemical assay. The inhibitive effect of ATP on the cell proliferation was determined by MTT method.Results The expression of P2Y2 was observed both in SACC-83 and SACC-LM cell lines. The cell proliferation of SACC-83 and SACC-LM was inhibited ... |
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| Keywords: | ATP |
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