Anti-angiogenic activity of methanol extract of Phellinus linteus and its fractions |
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| Authors: | Yeon Sil Lee Yoon Hee Kim Eun Kyung Shin Dae Hwan Kim Jae-Yong Lee Jin-Kyung Kim |
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| Institution: | a Center for Efficacy Assessment and Development of Functional Foods and Drugs, Hallym University, 39 Hallym daehakgil, Chuncheon 200-702, Republic of Korea
b Department of Biochemistry, College of Medicine, Hallym University, Chuncheon 200-702, Republic of Korea |
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| Abstract: | Aim of the studyThe aim of the present study was to investigate the effects of MeOH extract of PL (PLME) and its fractions on angiogenesis.Materials and methodsPLME and its subsequent fractions (methylene chloride, ethyl acetate, n-butanol and aqueous fractions) were evaluated in vitro. Specifically, the anti-angiogenic activities of PLME and its fractions were investigated by measuring their effects on the proliferation, migration, tube formation and phosphorylation of vascular endothelial growth factor receptor (VEGFR)-2 in human umbilical vein endothelial cells (HUVECs). In addition, the in vivo Matrigel plug model was applied to evaluate new vessel formation.ResultsThe results revealed that PLME and its subsequent fractions, except for the aqueous fraction, led to significant inhibition of the proliferation, migration, tube formation and VEGFR-2 phosphorylation of HUVECs as well as in vivo angiogenesis.ConclusionsThese findings indicate the potential for the use of PLME in pathological situations involving stimulated angiogenesis, such as inflammation and tumor development. |
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| Keywords: | PL Phellinus linteus PLME MeOH extract of Phellinus linteus HUVECs human umbilical vein endothelial cells VEGF vascular endothelial growth factor CAM chorioallantoic membrane |
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