Extracts from the Mongolian traditional medicinal plants Dianthus versicolorFisch. and Lilium pumilum Delile stimulate bile flow in an isolated perfused rat liver model |
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Authors: | Astrid Obmann Theresia Thalhammer Thanh Phuong Nha Vo Damdinsuren Natsagdorj Christa Kletter |
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Affiliation: | a Department of Pharmacognosy, University of Vienna, Althanstrasse 14, 1090 Vienna, Austria b School of Traditional Medicine, Health Sciences University of Mongolia, Ulaanbaatar, PO 48 Box 111, Mongolia c Institute of Pathophysiology, Medical University Vienna, General Hospital Vienna, Waehringer Guertel 18-20, 1090 Vienna, Austria d Health Sciences University of Mongolia, Ulaanbaatar, PO 48 Box 111, Mongolia e Otoch Manramba, Institute of Traditional Mongolian Medicine, Ulaanbaatar PO Box 49/235, Mongolia |
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Abstract: | Aim of the studyDianthus versicolor (Caryophyllaceae) and Lilium pumilum (Liliaceae) are two medicinal plants used in traditional Mongolian medicine to treat hepatic and gastrointestinal disorders. In this study aqueous (AE) and methanolic (ME) extracts of Dianthus versicolor and Lilium pumilum were investigated for their influence on the bile flow. The aqueous extracts of both plants were tested in absence and presence of 10 μM taurocholic acid at three different concentrations (100, 250, and 500 mg/L). The aqueous extract of Dianthus versicolor was further purified in order to locate the active principles. Two resulting fractions, one enriched in flavonoids and the other in sugars, were investigated for their influence on the bile flow in absence of taurocholic acid at 10, 20, and 40 mg/L. The aqueous extracts of both plants were analysed qualitatively by LC-MSn and quantitatively by UV-spectrophotometry.Materials and methodsThe bile flow experiments were performed in the isolated perfused rat liver. The compounds were identified by LC-DAD-MSn and TLC using references. The UV-spectrophotometric analysis was based on the monograph “Passiflorae herba” of the European Pharmacopoeia, and the total flavonoid contents were calculated and expressed as vitexin.ResultsAE and ME of both plants increased the bile flow dose-dependently (between 9% and 30%), and no hepatotoxic effect was seen even during longer perfusions. Stimulation of bile secretion was comparable in the presence and in the absence of taurocholic acid. The flavonoid fraction of Dianthus versicolor increased the bile flow by 18% (p < 0.05) at 40 mg/L, which was comparable to the positive control cynarin. The phytochemical investigations of the Dianthus versicolor AE (total flavonoid content 1.78%) revealed the presence of the isovitexin derivative saponarin. In the AE of Lilium pumilum (total flavonoid content 1.04%) the flavonoids rutoside, kaempferol-3-O-rutinoside, and isorhamnetin-3-O-rutinoside were detected.ConclusionsThe results show that choleresis under extract application is due to a stimulation of the bile-salt-independent bile flow which might be caused by the osmotic power of the extracts (hydrocholeresis). The flavonoids seem to contribute to the bile-flow-stimulating effect of Dianthus versicolor. Both plants exhibit a considerable choleretic effect that contributes to their use in traditional Mongolian medicine against gastrointestinal disorders. |
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Keywords: | Medicinal plants Dianthus versicolor Lilium pumilum Traditional Mongolian Medicine Isolated perfused rat liver Bile flow |
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