雄激素和雌激素受体与食管癌的关系

目的 通过测定食管癌组织中雄激素受体（AR）和雌激素受体（ER）的含量，探讨食管癌生物学行为与AR和ER的关系。方法 应用放射配体结合分析法定量测定31例食管癌患者癌组织中AR和ER的含量，并与正常食管黏膜组织作对照。结果 与正常食管组织比较，食管癌组织中存在较多的AR和ER，其含量与患者性别、分化程度、浸润程度、有无淋巴结转移有关（P＜0．05），而与患者年龄、肿瘤的部位无明显关系（P＞0．05）。结论 AR和ER含量与食管癌生物学行为关系密切，可能为食管癌的防治提供新的方法，并作为评价食管癌患者预后的新指标。

The Relationship Between Esophageal Cancer and Androgen-estrogen Receptors

Objective To study the effect of transfection exogenous PcDNA3 P16 expression plasmid on proliferation of human esophageal squamous carcinoma cell lines Ec109. Methods According to the different transfected plasmid and whether the transfection were underwent, three group were divided and 5 sample included in each group.P16 transfection group: p16 cDNA was introducted into Ec109 cell line by the vector of Lipofectamine. Empty plasmid transfection: PcDNA3 neo plasmid was introducted with the same method as a blank control group.Non transfection group: the Ec109 cell that were not transfected as a negative control group. Immuno fluorensence microscope and Northern dot hybirdization were used to detect P16 protein status. Methyl tetrazo thialum (MTT) method and flow cytometry were used for analysis of cell cycle and growth inhibition. Cell apoptosis was detected by DNA fragment electrophoresis and flow cytometry. Results The cell vitality of transfected Ec109 cells decreased and the G1 phase arrest detected after PcDNA3 P16 expression plasmid was transfected and expressed in Ec109 cell lines. Cell apoptosis was observed while P16 protein transient expressed, but the other control groups had negative results. Conclusion Transfection exogenous p16 gene to Ec109 conducted by liposome would result in cell growth inhibition. Transient p16 gene expression could induce cell apoptosis.