哮喘患者CD8+T细胞对单核/巨噬细胞抗原递呈功能的影响

目的：探讨支气管哮喘患者CD8⁺T细胞对单核/巨噬细胞抗原递呈功能的影响。方法：哮喘患者20例，健康对照22例，分别取静脉血5 mL，并分离MΦ、CD8⁺T细胞和B细胞。每份血样分成4组：MΦ递呈抗原组、CD8⁺T细胞参与MΦ递呈抗原组、CD8⁺T细胞体外活化后对MΦ递呈抗原影响组及自然状态下CD8⁺T细胞对MΦ细胞递呈抗原影响组。各组用CTLL₂P抗原刺激18 h后，洗去刺激原，与自体B细胞共同孵育10 d，吸取上清液，测定特异性IgM、IgE、IgG含量。结果:①哮喘患者MΦ单独递呈抗原，自体B细胞特异性IgM(A490值)（0.034±0.022）明显低于健康人(0.116±0.080)（P<0.05）；CD8⁺T细胞与MΦ共同培养递呈抗原时，产生的特异性IgM(A490值)(0.031±0.021)低于健康人(0.079±0.064)（P<0.05）；②哮喘患者CD8⁺T细胞与MΦ共同培养递呈抗原时，产生的特异性IgG(A490值)(0.102±0.041)明显高于健康人(0.081±0.067)（P<0.05），自然状态下及体外活化后CD8⁺T细胞与递呈抗原MΦ共同培养，产生的特异性IgG(A490值)(0.105±0.066, 0.079±0.059)与健康人(0.066±0.038, 0.069±0.047)无明显差别(P>0.05)；③哮喘患者CD8⁺T细胞与MΦ共同培养递呈抗原产生的特异性IgE(A490值)(0.171±0.154)高于健康人(0.147±0.059)（P<0.05）。结论:哮喘患者CD8⁺T细胞对MΦ递呈抗原产生免疫球蛋白有调节作用,而且参与哮喘发病。

Effects of CD8 + T lymphocytes on processing macrophages phagocytes of patients with asthma

Objective To study the effects of CD8 + T lymphocytes on processing macrophages (MΦ) of patients with asthma. Methods The MΦ and CD8 + T lymphocytes were isolated from peripheral blood of 20 patients with asthma and 22 health donors, respectively. Every sample was divided into four groups. All groups were stimulated with CTLL 2P antigen for 18 hours and CTLL 2P antigen were washed away, then they were cultured with B lymphocytes together. The levels of secreted CTLL 2P-specific IgM, IgG, and IgE in the supernatant were measured after 10 days. Results ①When MΦ processed antigen only, the level of CTLL 2P-specific IgM of patients with asthma(0.034±0.022) was lower than that of health donors (0.116±0.080) (P<0.05). The level of CTLL 2P-specific IgM of patients with asthma (0.031±0.021) was lower than that of health donors (0.079±0.064) (P<0.05),when CD8 + T lymphocytes were cultured with mononuclear phagocytes together. ②When CD8 + T lymphocytes were cultured with mononuclear phagocytes together, the level of CTLL 2P-specific IgG of patients with asthma (0.102±0.041) was significantly higher than that of health donors (0.081±0.067) (P<0.05). However, the IgG levels had not differences when active CD8 + T lymphocytes or natural CD8 + T lymphocytes were cultured with antigen processing mononuclear phagocytes (0.105±0.066,0.079±0.059),compared with health donors (0.066±0.038,0.069±0.047)(P>0.05). ③The CTLL 2P-specific IgE level of patients with asthma (0.171±0.154) was higher than that of health donors (0.147±0.059) (P<0.05), when CD8 + T lymphocytes were cultured with mononuclear phagocytes together to process antigen. Conclusion The CD8 + T lymphocytes of patients with asthma have different regulatory effect on immunolglobulin production from antigen processed by MΦ.