Transgenic mice carrying an Xist-containing YAC |
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Authors: | Heard E; Kress C; Mongelard F; Courtier B; Rougeulle C; Ashworth A; Vourc'h C; Babinet C; Avner P |
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Institution: | Unite de Genetique Moleculaire Murine, Institut Pasteur, Paris, France. |
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Abstract: | The initiation of X-chromosome inactivation in female mammals is controlled
by a key locus, the X-inactivation centre (Xic). The Xist gene, which maps
to the candidate region for Xic and is expressed exclusively from the
inactive X chromosome, is thought to be an essential component of the Xic.
To test whether sequences spanning several hundred kilobases and including
Xist from the Xic region are capable of initiating inactivation, we have
created a series of transgenic mice using a 460 kb yeast artificial
chromosome (YAC). Analysis in these mice of the expression of Xist, of a
LacZ reporter gene and of two genes in the region that are normally silent
on the inactive X chromosome, suggests that essential sequences for Xist
expression and X-inactivation may be absent in these transgenic animals.
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