成人急性白血病患者FLT3基因及FLT3/ITD突变

目的 研究FLT3基因及FLT3基因内部串联重复(internal tandem duplication,ITD)突变(FLT3/ITD突变)与蒙古族成人急性白血病的关系,探索其在急性白血病的诊断及基因分型中的意义.方法 采用聚合酶链反应(PCR)技术检测56例蒙古族成人初发急性白血病、10例蒙古族成人非白血病患者FLT3基因表达及FLT3/ITD突变情况.结果 41例初诊急性髓细胞白血病(AML)患者中FLT3基因表达为70.7%(29/41);FLT3/ITD突变阳性率为19.5%(8/41).15例初诊急性淋巴细胞白血病(ALL)患者中FLT3基因表达为60.0%(9/15),15例ALL患者均未检测到FLT3/ITD基因突变.10例对照均无FLT3基因表达.结论 FLT3基因在蒙古族成人急性白血病患者中有较高表达,FLT3基因的检出可能有助于急性白血病的诊断.ALL患者一般无FLT3/ITD突变,推测FLT3/ITD突变检测有助于急性白血病基因分型.

FLT3 gene and FLT3/ITD mutation in adult acute leukemia

Objective To analyze the relationship between FLT3 gene and FLT3 gene internal tandem duplication mutation (FLT3/ITD) with Mongolian adult acute leukemia, and explore the significance of FLT3 gene and FLT3/ITD in diagnose and genotyping of acute leukemia. Methods PCR was used to detect the FLT3 gene and the FLT3/ITD in 56 Mongolian adult acute primary leukemia patients and 10 Mongolian adult acute non-leukemia patients. Results The expression of FLT3 gene was detected in 70.7 (29/41) primary acute myeloid leukemia (AML) cases and 19.5% (8/41) was found with FLT3/ITD mutation.FLT3 gene expression in 15 primary acute lymphoblastie leukemia (ALL) was also found a high level of 60.0% (9/15).FLT3/ITD mutation was not found in ALL patients. There was no FLT3 gene expression in control grope. Conclusion For a high level of the FLT3 gene expression in Mongolian adult acute leukemia,FLT3 gene detection might be helpful for diagnosing acute leukemia.FLT3/ITD mutation might be helpful to identify the genie characterization of acute leukemia.