人胎盘源间充质干细胞对脐血单核源树突状细胞体外成熟的调节作用

目的探讨人胎盘源间充质干细胞（PMSCs）对单核源树突状细胞（mono-DCs）体外成熟的影响。方法采用密度梯度离心法分离人脐血单核细胞,在人粒细胞-巨噬细胞集落刺激因子（hGM-CSF）和人白细胞介素-4（hIL-4）刺激下获得大量未成熟DCs。将DCs和PMSCs共培养4 d,同时加入LPS刺激,分别通过倒置显微镜、流式细胞仪和混合淋巴反应（MLR）检测DCs的成熟。结果共培养DCs呈散在分布,细胞边缘圆滑;与成熟DCs相比,其表面CD80、CD86、CD83、CD40的表达明显较低,而CD14的表达较高;共培养组DCs刺激同种异型淋巴细胞增殖的能力在各个浓度均明显低于对照组（均P〈0.05）。结论 PMSCs可以明显抑制脐血单核源DCs的体外成熟。

Effects of Human Placenta-derived Mesenchymal Stem Cells on Regulation against the Maturation of Cord Blood Monocyte-derived Dendritic Cells in vitro

Objective To study on the in vitro maturation of cord blood monocyte-derived dendritic cells（mono-DCs） regulated by human placenta-derived mesenchymal stem cells（PMSCs）.Methods Human cord blood monocytes were isolated by density gradient centrifugation and cultured with human granulocyte-macrophage colony stimulating factor（hGM-CSF） and human interleukin-4（hIL-4）.Then large amounts of immature DCs were obtained and co-cultured with PMSCs in the presence of LPS for 4 days.The effects of PMSCs on the maturation of DCs were analyzed with inverted microscope,flow cytometry and mixed lymphocyte reaction respectively.Results In co-culture group,DCs were scattering around and presented round and smooth round and under inverted microscope.Compared with the control group,co-cultured DCs expressed lower level of CD80,CD86,CD83 and CD40,but higher level of CD14.Allogeneic lymphocytes proliferation assay showed that the stimulatory abilities of DCs from co-culture group were lower than those in the control group（all P〈0.05）.Conclusion PMSCs can significantly suppress the maturation of cord blood mono-DCs in vitro.