p14ARF、Gadd45a mRNA在甲状腺乳头状癌中的表达及其临床意义

目的研究甲状腺乳头状癌组织中p14ARF、Gadd45a mRNA及蛋白的表达。方法采用RT-PCR及免疫组织化学技术检测56例甲状腺乳头状癌、20例乳头状微小癌和20例正常甲状腺组织中Gadd45a、p14ARF的mRNA及蛋白的表达。结果 Gadd45a mRNA在甲状腺乳头状癌、乳头状微小癌和正常甲状腺组织的阳性表达率分别为66.1%（37/56）、60.0%（12/20）和25.0%（5/20）。p14ARFmRNA在甲状腺乳头状癌、乳头状微小癌和正常甲状腺组织中的阳性表达率分别为39.3%（22/56）、60.0%（12/20）和95.0%（19/20）。Gadd45a mRNA在甲状腺乳头状癌、乳头状微小癌组织中的阳性表达率明显高于正常甲状腺组织（均P〈0.05）。p14ARFmRNA在甲状腺乳头状癌、乳头状微小癌组织中的阳性表达率明显低于正常甲状腺组织（均P〈0.05）。p14ARFmRNA在甲状腺乳头状癌组织中的阳性表达率明显低于乳头状微小癌组织（P〈0.05）。免疫组化检测二者蛋白的表达结果与mRNA检测结果相同。在复发的甲状腺乳头状癌组织中Gadd45a mRNA的阳性表达率明显高于未复发的甲状腺乳头状癌（P〈0.05）。在有淋巴结转移的甲状腺乳头状癌组织中p14ARFmRNA的阳性表达率明显低于无淋巴结转移者（P〈0.05）。Gadd45a与p14ARFmRNA表达无相关性（P〉0.05）。结论 p14ARF、Gadd45a可能与甲状腺乳头状癌的发生及进展有一定相关性,联合检测二者的表达可为甲状腺乳头状癌的早期诊断提供依据,并有助于判断预后。

Expressions of p14ARF and Gadd45a mRNA in Thyroid Papillary Carcinoma and Its Clinical Significance

Objective To study the expression and clinical significance of Gadd45a,p14ARF mRNA in thyroid papillary carcinoma.Methods RT-PCR and immunohistochemical staining were used to dert-ermine the expression of p14ARF and Gadd45a in 56 patients with thyroid papillary carcinoma,20 patients with thyroid micropapillary carcinoma and 20 cases of nontumorous thyroid tissure.Results The positive incidence of Gadd45a mRNA expression in thyroid papillary carcinoma,thyroid micropapillary carcinoma and nontumorous thyroid tissure was 66.1%（37/56）,60.0%（12/20）,and 25.0%（5/20） respectively. The difference was significant（P〈0.05）.The positive incidence of pl4 mRNA expression in thyroid papillary carcinoma,thyroid micropapillary carcinoma and nontumorous thyroid tissure was 39.3% （22/56）,60.0%（12/20）,and 95.0%（19/20）.The difference was significant（P〈0.05）.The positive incidence of pl4 mRNA expression was much lower in thyroid papillary carcinoma than that in thyroid micropapillary carcinoma（P〈0.05）.The positive incidence of Gadd45a protein and pl4 protein detected by immunohistochemical staining had the same results.In recurring the positive incidence of Gadd45a mRNA was much higer than that without recurring（P〈0.05）.In lymph node metastasis the positive incidence of p14ARF mRNA was much lower than that in lymph node without metastasis（P 0.05）.There was no correlation between positive incidence of Gadd45a mRNA and p14ARF mRNA（P 0.05）.Conclusion The Gadd45a and p14ARF play an importmant role in growth and invasion of thyroid papillary carcinoma.Expression of Gadd45a and pl4 may prove to be valuable in treatment and prognosis of thyroid papillary carcinoma.