| 姜黄素对大鼠肝脏冷缺血再灌注损伤的保护作用及其机制 |
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| 引用本文： | 安伟|刘军|管冬诗|杨凤辉|许世峰|周旭. 姜黄素对大鼠肝脏冷缺血再灌注损伤的保护作用及其机制[J]. 中国普通外科杂志, 2010, 19(1): 32-36 |
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| 作者姓名： | 安伟|刘军|管冬诗|杨凤辉|许世峰|周旭 |
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| 作者单位： | 山东大学附属省立医院器官移植中心肝胆外二科; |
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| 基金项目： | 山东省优秀中青年奖励基金资助项目(2005BS03006) |
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| 摘 要： | 目的探讨姜黄素预处理对大鼠肝脏冷缺血再灌注损伤(IRI)的保护作用及其机制。方法将40只Wistar大鼠随机分为:实验组(21只),于术前2 h将60 mg/kg的姜黄素溶于1mL DMSO中静脉注射;对照组(19只),于术前2h静脉注射1 mL DMSO。肝脏冷灌注时间为30 min,恢复血供复流6 h后处死动物,留取血液和肝脏标本,行血清ALT,AST,LDH和组织匀浆SOD,MDA,MPO测定,并进行组织病理和细胞凋亡检测。酶联免疫吸附试验(ELISA)检测肝组织匀浆中TNF-α及MIP-2的水平变化。结果实验组大鼠血清ALT,AST,LDH水平明显低于对照组(P0.01);实验组MDA,MPO,TNF-α和MIP-2水平较对照组明显降低(P0.05),SOD含量较对照组明显增高(P0.05)。并且实验组大鼠肝组织损伤程度和细胞凋亡程度明显轻于对照组。结论姜黄素预处理能减轻大鼠肝脏冷IRI,其保护机制可能与提高肝组织SOD含量,抑制脂质过氧化与细胞凋亡,下调炎性因子TNF-α和MIP-2的表达以及减少中性粒细胞浸润有关。
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| 关 键 词： | 再灌注损伤； 肝； 姜黄素； 大鼠 |
| 收稿时间： | 2009-10-10 |
| 修稿时间： | 2009-12-14 |
The protective effect of curcumin in hepatic cold ischemia-reperfusion injury in rats |
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| AN Wei,LIU Jun|GUAN Dongshi,YANG Fenghui,XU Shifeng,ZHOU Xu. The protective effect of curcumin in hepatic cold ischemia-reperfusion injury in rats[J]. Chinese Journal of General Surgery, 2010, 19(1): 32-36 |
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| Authors: | AN Wei LIU Jun|GUAN Dongshi YANG Fenghui XU Shifeng ZHOU Xu |
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| Affiliation: | AN Wei,LIU Jun,GUAN Dongshi,YANG Fenghui,XU Shifeng,ZHOU Xu(Organ Transplantation Center,the Second Department of Hepatobiliary Surgery,Affiliated Provincial Hospital,Sh,ong University,Jinan 250021,China) |
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| Abstract: | Objective To investigate the protective effect of curcumin in hepatic cold ischemia-reperfusion injury in rats and its possible mechanisms.Methods Forty Wistar rats were randomly divided into two groups: the experimental group(n=21),rats were administered curcumin by injection through tail vein at the dose of 60mg/kg which was dissolved in one milliliter DMSO two hours before cold ischemia period,and the remaining rats served as control group.The hepatic cold ischemia was 30 minutes.All the rats were sacrif... |
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| Keywords: | Reperfusion Injury Liver Curcumin Rats |
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