| 人参栽培土壤中细菌种群ARDRA研究体系的建立 |
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| 引用本文： | 应益昕,丁万隆,李勇.人参栽培土壤中细菌种群ARDRA研究体系的建立[J].中国中药杂志,2011,36(3):366-369. |
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| 作者姓名： | 应益昕 丁万隆 李勇 |
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| 作者单位： | 中国医学科学院北京协和医学院药用植物研究所,北京,100193 |
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| 基金项目： | 高等学校博士学科点专项科研基金项目(200800231060) |
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| 摘 要： | 目的:建立适用于开展人参栽培土壤微生物种群结构分析的ARDRA研究体系.方法:从基因组水平研究人参栽培土壤微生物种群多样性结构.结果:对人参栽培土壤样品分析发现,ARDRA体系不仅能高效区分土壤中不同的微生物类群,而且还可根据酶切谱带的差异统计出不同微生物类群在土壤总微生物中所占的比重.结论:建立的ARDRA研究体系能够用于人参栽培土壤微生物种群结构分析.
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| 关 键 词： | 人参 栽培土壤 细菌 ARDRA |
| 收稿时间： | 2010/10/24 0:00:00 |
Establishment of ARDRA system for Panax ginseng cultivated soil microbial community study |
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| YING Yixin,DING Wanlong and LI Yong.Establishment of ARDRA system for Panax ginseng cultivated soil microbial community study[J].China Journal of Chinese Materia Medica,2011,36(3):366-369. |
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| Authors: | YING Yixin DING Wanlong and LI Yong |
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| Institution: | Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China;Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China;Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China |
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| Abstract: | In this study, ARDRA system was established for Panax ginseng cultivated soil microbial community analysis. In the process of soil analysis we found that, ARDRA can not only distinguish soil microbial communities, proportion of each microbial type in total microorganisms can be calculated based on profiles of restricted enzyme digested 16S rDNA, also. Results indicated that, ARDRA system established was able to analyze microbial communities of P. ginseng cultivated soil samples. |
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| Keywords: | Panax ginseng cultivated soil bacteria ARDRA |
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