| RP-HPLC法测定灵芝胶囊中腺苷的含量 |
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| 引用本文： | 李开志,雷灼雨,巴国际. RP-HPLC法测定灵芝胶囊中腺苷的含量[J]. 中国药房, 2007, 18(30): 2371-2372 |
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| 作者姓名： | 李开志 雷灼雨 巴国际 |
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| 作者单位： | 1. 重庆垫江县人民医院,垫江县,408300
2. 重庆市药品检验所,重庆市,401121 |
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| 摘 要： | 目的:建立以反相高效液相色谱法测定灵芝胶囊中腺苷含量的方法。方法:色谱柱为Diamonsil C18(150mm×4.6mm,5μm),流动相为甲醇-0.01mol.L-1磷酸二氢钾溶液(10∶90),流速为0.8mL.min-1,测定波长为260nm,柱温为30℃。结果:腺苷进样量在0.0814~2.0360μg范围内与峰面积积分值呈良好线性关系(r=0.9994);平均回收率为99.37%,RSD=0.23%(n=6)。结论:本方法准确、简便、快速、可靠,可用于灵芝胶囊的质量控制。
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| 关 键 词： | 灵芝胶囊 腺苷 反相高效液相色谱法 含量测定 |
| 文章编号： | 1001-0408（2007）30-2371-02 |
| 收稿时间： | 2006-12-12 |
| 修稿时间： | 2007-04-04 |
Determination of Adenosine in Ganoderma Capsule by RP - HPLC |
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| LI Kaizhi,LEI Zhuoyu,BA Guoji. Determination of Adenosine in Ganoderma Capsule by RP - HPLC[J]. China Pharmacy, 2007, 18(30): 2371-2372 |
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| Authors: | LI Kaizhi LEI Zhuoyu BA Guoji |
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| Abstract: | OBJECTIVE:To establish a RP-HPLC method for the determination of Adenosine in Ganoderma capsule.METHODS:HPLC method was adopted with Diamonsil C18(150mm× 4.6mm,5μ m)at a temperature of 30℃.The mobile phase consisted of methanol-0.01mol· L-1 KDP solution(10∶ 90)at a flow rate of 0.8mL· min-1.A UV-detector at 260nm was used.RESULTS:The linear range of Adenosine was 0.081 4～ 2.036 0μ g(r=0.999 4).The average recovery was 99.37%(RSD=0.23%,n=6).CONCLUSION:The method is accurate,simple,rapid,and reliable,and suitable for the quality control of Ganoderma capsule. |
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| Keywords: | Ganoderma capsule Adenosine RP-HPLC Content determination |
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