缺血再灌注对大鼠学习记忆及海马神经元Glu、NR1和NR2B表达的影响

目的观察脑缺血再灌注对大鼠学习记忆能力及海马神经元谷氨酸（Glu）、NR1、NR2B表达的影响，探讨脑缺血损伤与Glu兴奋性毒性的关系。方法采用PULLINSIN-4VO改良法制备血管性痴呆动物模型，MORRIS水迷宫检测其空间学习记忆能力，苏木精-伊红染色显示海马组织结构变化，免疫组织化学SABC法显示海马神经元Glu及NR1、NR2B的表达。结果缺血15min再灌注损伤48h后，海马神经元大量坏死损伤，大鼠学习记忆能力明显下降，海马内Glu、NR1和NR2B表达显著增加（F=22．063～1368．839，q=3．453～5．342，P〈0．01）。结论缺血再灌注使大鼠学习记忆能力下降，其机制与海马神经元Glu、NR1和NR2B的表达增加有关。

CHANGES OF LEARNING AND MEMORY ABILITIES AND EXPRESSION OF HIPPOCAMPUS Glu, NR1 AND NR2B ON CEREBRAL ISCHEMIC REPERFUSION INJURY IN RATS

ObjectiveTo observe the changes of learning and memory abilities and effects of expression of glutamate neutrons(Glu),NMDA receptor subunit NR_1 and NR_(2B) in hippocampus glutamate neutrons of rats with cerebral ischemic reperfusion(IR),and to study the relationship between cerebral ischemia damage and glutamate excitability toxicity.MethodsThe improved PULLINSIN-4VO was applied to produce the models of vascular dementia,and MORRIS Maze test for the ability of space learning and memory,the staining of hippocampus tissue structure was taken by HE staining method.Immunohistochemical SABC staining and image analysis were used to present the expression of Glu,NR_1 and NR_(2B) in hippocampus.ResultsThe necrosis of hippocampus neutrons of rats occurred obviously after ischemia for 15 min and IR 48h,and the expression of Glu,NR_1 and NR_(2B) in hippocampus neutrons significantly increased in the hippocampus,and learning and memory abilities were obviously lowered(F=22.063-1 368.839,q=3.453-5.342,P<0.01).ConclusionThe expressions of Glu,NR_1 and NR_(2B) were enhanced after cerebral ischemia and reperfusion.The decline of learning and memory abilities is related to the increase of expression of Glu,NR_1 and NR_(2B) in hippocampus.