四物汤改善多囊卵巢综合征大鼠卵巢功能实验研究

目的 观察四物汤(Siwu tang,SWT)对多囊卵巢综合征(polycystic ovarian syndrome,P-COS)大鼠动情周期、卵巢形态学及血清激素水平的影响.方法 选取SPF级雌性Wistar大鼠25只,随机分为对照组、模型组、SWT低剂量组、SWT高剂量组和二甲双胍组,每组5只.除对照组外,各实验...

Experimental Study of Siwu Tang on the Improvement of Ovarian Function in Rats with Polycystic Ovary Syndrome

Objective To observe the effect of Siwu Tang(SWT)on the estrous cycle,ovarian morphology and serum hormone levels in polycystic ovary syndrome(PCOS)rats.Methods Twenty-five SPF female Wistar rats were randomly divided into control group,model group,SWT low-dose group,SWT high-dose group and metformin group,with 5 rats in each group.Except for the control group,rats in each experimental group were gavage with 1 mg/(kg·d)letrozole to establish PCOS rat models.After successful modeling,the rats in SWT low-dose group were gavage with 8.64 g/(kg·d)SWT,SWT high-dose group were gavage with 25.92 g/(kg·d)SWT,metformin group were gavage with 200 mg/(kg·d)metformin,while the control and model group were gavage with the same dose of 0.9%NaCl injection.After 4 weeks of continuous intervention in each group,the estrus cycle of each group were recorded,the ovarian morphology were observed,and the serum hormone level were detected.Results Compared with control group,the time of estrus in the model group were significantly shortened(P<0.05),the number of corpus luteum were significantly decreased,the number of cysts were significantly increased(P<0.05),the serum levels of testosterone(T),luteinizing hormone(LH)and the LH/FSH ratio were significantly increased,the levels of estradiol(E2)and follicle-stimulating hormone(FSH)were significantly reduced.Compared with the rats in the model group,the time of estrous in the SWT low-dose group,SWT high-dose group and metformin group were significantly increased(P<0.05),the number of corpus luteum were significantly increased,and the number of cysts were significantly reduced(P<0.05),the serum levels of T and the LH/FSH ratio were significantly decreased(P<0.05).The serum levels of E2 rats in the SWT low-dose group,SWT high-dose group were significantly increased(P<0.05).The serum levels of FSH in the SWT high-dose group were significantly increased,and levels of LH were significantly decreased(P<0.05).Conclusion SWT could increase the time of estrus,reduce ovarian cysts,increase the number of corpus luteum,reduce the serum levels T,LH and LH/FSH ratios,and increase E2 and FSH levels in PCOS rats.The therapeutic mechanism might be related to improvement of ovarian function in PCOS rats by regulating serum hormone levels.