RP-HPLC法测定人血浆中氟吡汀的浓度

目的建立以HPLC法测定人血浆中马来酸氟吡汀的浓度的方法。方法采用DiamonsilC18色谱柱,流动相:5 mmol.L-1磷酸氢二钠缓冲溶液-乙腈(体积比为55∶45),流速:1.0 mL.min-1,荧光检测激发波长:323 nm,发射波长:370 nm。结果氟吡汀血药质量浓度在10.0~1 500.0μg.L-1内线性关系良好(r=0.999),定量下限为10μg.L-1,方法回收率平均为105.36%,提取回收率平均为90.74%,日内、日间RSD均小于10%。结论本方法适用于人血浆中氟吡汀的药代动力学研究。

Determination of the concentration of flupirtine in human plasma by RP-HPLC

Objective To establish an HPLC method for the determination of flupirtine in human plasma．Methods The separation was carried on a Diamonsil C₁₈ column with the mobile phase consisted of 5 mmol·L^-1 phosphate buffer -acetonitrile (V:V=55:45); the flow rate was 1.0 mL·min^-1 and the excitation and emission wavelengths were set at 323 and 370 nm, respectively. Results The linear range of flupirtine was 10.0-1500.0 μg·L^-1 (r=0.999), with the lower quantity limit of 10.0 μg·L^-1．The average method recovery was 105.36％ and the average extraction recovery was 90.74％．The inter-day RSD and intra-day RSD were both less than 10％．Conclusions The method is simple, sensitive, rapid and accurate, and can be used for the pharmacokinetic study of flupirtine．