Phytochemical analysis and in vitro antioxidant acitivity of hydroalcoholic seed extract of Nymphaea nouchali Burm. f. |
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Authors: | Mabel Parimala Francis Gricilda Shoba |
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Institution: | 1.P.G. & Research Department of Zoology, Voorhees College, Vellore, Tamilnadu, India;Associate Professor, Dept. of Biotechnology, School of Life Sciences, Pondicherry University, Pudhucherry- 605014, India., Tel: 0413-2654745, 09443135842, E-mail: |
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Abstract: | ObjectiveTo evaluate the phytochemical constituents and the antioxidant activity of hydroalcoholic extract of Nymphaea nouchali seed locally prescribed as a diet for diabetes mellitus.MethodsThe antioxidant and free radical scavenging activity of hydroalcoholic extract of the plant was assessed against 1,1 diphenyl-2-picryl hydrazyl (DPPH), nitric oxide and lipid peroxidation using standard protocols. Total phenolics, flavonoids and tannins were also determined.ResultsPhytochemical analysis revealed the presence of phenols, flavones, tannins, protein, reducing sugars, glycosides, saponins, alkaloids and steroids. The activities of plant extract against DPPH, nitric oxide and lipid peroxidation was concentration dependent with IC50 value of 42.82, 23.58 and 54.65 µg/mL respectively. The total antioxidant capacity was high with 577.73 mg vitamin E/g of the extract and showed a moderately high vitamin C content of 197.22 mg/g. The total tannin content of hydroalcoholic seed extract was high (195.84 GE/g), followed by phenolics (179.56 GE/g) and flavonoids (23.55 QE/g).ConclusionOur findings provide evidence that the crude extract of Nymphaea nouchali is a potential source of natural antioxidants and this justifies its use in folkloric medicine. |
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Keywords: | Nymphaea stellata Phytochemical Antioxidant Scavenging Free radicals DPPH Nitric oxide Lipid peroxidation Phenols Flavonoids |
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