An ELISA that detects cells-associated and released rat IL-2 receptors in a soluble form |
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Authors: | Athanasia Mouzaki Hisao Osawa Tibor Diamantstein |
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Affiliation: | Immunology Research Unit, Klinikum Steglitz, Freie Universität Berlin, West Berlin, F.R.G. |
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Abstract: | A mouse anti-rat interleukin-2 receptor (IL-2R) monoclonal antibody (mAb), ART-65, has recently been developed which recognizes the rat IL-2R but binds to an epitope distinct from that recognized by the mAb ART-18. The availability of two mAb against the rat IL-2R molecule permitted the development of an enzyme-linked immunosorbent assay (ELISA) which detects soluble and solubilized rat IL-2R. Using this ELISA, time course studies of ConA and LPS-activated rat splenocytes revealed that IL-2R are released into their environment during the culture period, and that IL-2R can be detected in rat sera. The significance of the results is discussed. |
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Keywords: | ELISA Interleukin-2 receptor Monoclonal antibody (Rat) |
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