芍药甘草汤配伍意义的药动学研究

目的：通过检测给予芍药、甘草单方或合剂芍药甘草汤后，动物血浆中芍药苷、甘草次酸的药动学参数，探索芍药甘草汤配伍的合理性。方法：将芍药、甘草及芍药甘草汤制成水煎液，分别灌胃给予SD大鼠，采用HPLC／MS／MS法测定大鼠血浆中芍药苷、甘草次酸的血药浓度，并计算主要药动学参数。结果：与单方相比，给予芍药甘草汤后大鼠血浆中甘草次酸的达峰时间提前，峰浓度增加；芍药苷达峰浓度提高、相对生物利用度增加；两成分均出现半衰期缩短现象。结论：臣药甘草促进了君药芍药中芍药苷的吸收，提高了它的体内浓度与含量，这可能是复方芍药甘草汤解痉、镇痛、镇静等功效强劲有力的体内药动学依据。君药芍药提前助燃了臣药甘草中甘草次酸在体内出现的时间和数量，这也可能是复方芍药甘草汤抗溃疡、解痉、抗炎等功效迅速高效的体内药动学依据。本实验从药动学角度证明了芍药甘草汤配伍的合理性。

Pharmacokinetic Study of Herbaceous Compatibility of Peony Liquorice Decoction

The study is designed to understand the rationality of herbaceous compatibility of a peony liquorice decoction through analyzing the pharmacokinetic parameters of peoniflorin and glycyrrhetinic acid in SD rats plasma. A HPLC/ MS/MS method was used to detect the concentration of peoniflorin and glycyrrhetinic acid in SD rats administrated with peony, liquorice or peony liquorice decoction. Results show that peoniflorin and glycyrrhetinic acid in peony liquorice decoction have a short Tpeak, a high Cmax, and a short T1/2. Comparing with the sole administration of peony or liquorice, the peony liquorice decoction was able to make glycyrrhetinic acid in rat plasma reach the peak concentration in a faster manner, with an increased Cmax. Peoniflorin＇s peak concentration got enhanced, and so did the relative bioavailability. Both the ingredients have showed a reduced half-life. It is believed that liquorice facilitates the absorption of peoniflorin in peony, and enhances in vivo concentration, which is possibly associated with the pharmacokinetic effects of peoniflorin, including spasmolysis, analgesia, and tranquilization. Peony promotes the releases of glycyrrhetinic acid in liquorice, in terms of both time and quantity, allowing an enhanced anti-ulcer, spasmolysis, and anti-inflammatory effect. The experiment has proved the rationality of peony liquorice decoction compatibilities in the context of pharmacokinetics.