载脂蛋白E基因多态性与中国人2型糖尿病肾脏并发症的相关性研究

目的 研究ApoE基因多态性与中国人2型糖尿病及其肾脏并发症（DN）的相关性。方法 以ApoE基因为候选基因,应用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）分析法检测78例糖尿病患者和49例健康体检者的ApoE基因型,其中合并肾脏并发症40例,无肾脏病变（DRD）38例。结果 DN组ApoE的ε2等位基因频率明显高于DRD组和正常对照组（P〈0.05）。DM患者的年龄、病程、高血压、GHB及ApoEε2等位基因频率与DN关系的Logistic回归分析：病程和ApoE的ε2等位基因与DN的发生密切相关（P〈0.05）,病程对DN的OR值为1.1494,而ApoE的ε2等位基因对DN的OR值为7.6514。结论 ApoE的ε2等位基因是2型DM并发DN的遗传易感因子。

Study on the relationship between apolipoprotein E(Apo E)gene polymorphism and Chinese patients with type 2 diabetes mellitus and diabetogenous nephropathy(DN)

Objective To investigate the relationship between apolipopretein E（ Apo E）gene polymorphism and Chinese patients with type 2 diabetes mellitus and diabetic nephropathy（DN） . Methods Apo E genotype was determined by PCR - RFLP in 78 diabetes（38 without DN ,40 with DN） and 49 non - diabetes as control. Results Significant heterogeneity was seen in Apo E genotypes between DN group and healthy control group （ P 〈 0.05）, so as between DN group and DRD group （ P 〈 0.05）. After the effects of other factors such as duration of history, age, GHB and hypertension were eliminated by Logistic regression, it was suggested that course of disease and Apo E e2 allele were closely related to the onset of DN （ P 〈 0.05）. The OR of course of disease to DN was 1. 1494, while the OR Apo E e2 allele to DN was 7. 6514. Condusions Apo E e2 allele is a genetic susceptible factors to type 2 diabetic nephropathy.