| 透明质酸寡糖逆转白血病细胞系K562/ADR多药耐药的研究 |
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| 引用本文： | 崔向珍,郑雪凌,孙静,马玉贞,王凤山.透明质酸寡糖逆转白血病细胞系K562/ADR多药耐药的研究[J].齐鲁药事,2012(10):568-570,576. |
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| 作者姓名： | 崔向珍 郑雪凌 孙静 马玉贞 王凤山 |
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| 作者单位： | [1]山东商业职业技术学院生物工程学院,山东济南250103 [2]山东大学药学院生化与技术药物研究所,山东济南250012 |
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| 摘 要： | 目的研究透明质酸寡糖(o-HAs)的体外逆转白血病多药耐药细胞(K562/A02)的耐药性,并对其逆转机制进行探讨。方法以四甲基偶氮唑盐微量酶反应比色法(MTT法)检测o-HAs对K562/A02细胞的多药耐药逆转活性;以流式细胞术检测o-HAs对K562/A02细胞内抗癌药物盐酸多柔比星(ADR)累积的影响。结果在100μg.mL-1浓度时o-HA4、o-HA6、o-HA8、o-HA10使ADR对K562/A02的半数抑制浓度(IC50)由61.6μg.mL-1分别降低至30.17、30.04、32.29、33.33μg.mL-1。细胞中ADR的累积量增加至原来的3.90、3.92、3.76、3.39倍。结论 o-HAs在体外具有较强的逆转K562/A02细胞多药耐药的活性。
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| 关 键 词： | 透明质酸寡糖 逆转 多药耐药性 |
Study on multi - drug resistance of leukemic cell K562/AO2 reversed by oligosaccharides of hyaluronan |
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| CUI Xiang-zhen,ZHENG Xue-ling,SUN Jing,MA Yu-zhen,WANG Feng-shan.Study on multi - drug resistance of leukemic cell K562/AO2 reversed by oligosaccharides of hyaluronan[J].qilu pharmaceutical affairs,2012(10):568-570,576. |
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| Authors: | CUI Xiang-zhen ZHENG Xue-ling SUN Jing MA Yu-zhen WANG Feng-shan |
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| Institution: | 1. College of Bioengineering ,Shandong Institute of Commerce and Technology ,Jinan 250103,China ;2. Institute of Biochemical and Biotechnological Drugs, School of Pharmaceutical Science, Shangdong University, Jinan 250012, China ) |
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| Abstract: | Objective To study the drug resistance of leukemic cell K562/AO2 reversed by oligosaccharides of hyaluronan and to investigate the mechanism of its reverse effect. Methods The reverse effect of o - HAs on multi - drug resistance of leukemic cell K562/AO2 was detected by MIT method. The effect of o - HAs on adriamycin accumulated in K562/AO2 cell was detected by flow cytometry. Results The ICs0 of ADR decreased from 61.6 μg . mL^-1 to 30.17,30.04,32.29,33.33 μg ·mL^-1 after 100 μg.mL^-1 of o - HA4, o - HA6, o - HA8, o - HA10 were added respectively. The ADR accumulation in K562/AO2 cell was enhanced by 3.90,3.92,3.76 and 3.39 folds. Conclusion o - HAs has strong reverse effect on muli - drug resistance in K562/AO2. |
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| Keywords: | Oligosaccharides of hyaluronan Reverse Multi - drug resistance |
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