Production and characterization of monoclonal antibody to botulinum neurotoxin type B light chain by phage display |
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| Authors: | Lee Myung-Shin Lee Ji Chul Choi Cha Yong Chung Junho |
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| Affiliation: | Department of Microbiology and Immunology, Eulji University School of Medicine, Daejeon, South Korea. |
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| Abstract: | A monoclonal antibody to the light chain of botulinum neurotoxin type B (BoNT/B) was generated and its protective activity was evaluated in vivo. A chimeric rabbit/human Fab library was generated using bone marrow and spleen cDNAs of rabbits immunized with the BoNT/B light chain, and three monoclonal antibodies specific to the catalytic domain of BoNT/B were isolated. One of these clones, BCXRH1, was specific to a conformation-dependent epitope, and partially neutralized the BoNT/B complex in vivo. |
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