| 肉苁蓉提取工艺及干燥方式研究 |
| |
| 引用本文： | 黄翔,郭晔红,贾存勤,马旭东,杨丽平,黄广斌,阚建红. 肉苁蓉提取工艺及干燥方式研究[J]. 中草药, 2019, 50(15): 3622-3630 |
| |
| 作者姓名： | 黄翔 郭晔红 贾存勤 马旭东 杨丽平 黄广斌 阚建红 |
| |
| 作者单位： | 甘肃农业大学农学院, 中药材研究所, 甘肃省中药材规范化生产技术创新重点实验室, 甘肃省药用植物栽培育种工程中心, 甘肃省干旱生境作物学重点实验室, 甘肃兰州 730070,甘肃农业大学农学院, 中药材研究所, 甘肃省中药材规范化生产技术创新重点实验室, 甘肃省药用植物栽培育种工程中心, 甘肃省干旱生境作物学重点实验室, 甘肃兰州 730070,甘肃汇勤生物科技有限公司, 甘肃 白银 730600,甘肃农业大学农学院, 中药材研究所, 甘肃省中药材规范化生产技术创新重点实验室, 甘肃省药用植物栽培育种工程中心, 甘肃省干旱生境作物学重点实验室, 甘肃兰州 730070,甘肃农业大学农学院, 中药材研究所, 甘肃省中药材规范化生产技术创新重点实验室, 甘肃省药用植物栽培育种工程中心, 甘肃省干旱生境作物学重点实验室, 甘肃兰州 730070,甘肃农业大学农学院, 中药材研究所, 甘肃省中药材规范化生产技术创新重点实验室, 甘肃省药用植物栽培育种工程中心, 甘肃省干旱生境作物学重点实验室, 甘肃兰州 730070,甘肃农业大学农学院, 中药材研究所, 甘肃省中药材规范化生产技术创新重点实验室, 甘肃省药用植物栽培育种工程中心, 甘肃省干旱生境作物学重点实验室, 甘肃兰州 730070 |
| |
| 基金项目： | 国家自然科学基金资助项目（31860349）；国家自然科学基金资助项目（31860102）；甘肃省科技计划资助项目（18YF1NA072） |
| |
| 摘 要： | 目的选择肉苁蓉最佳提取工艺参数,研究不同干燥方式对5种苯乙醇苷类成分的影响。方法采用单因素筛选结合Box-Behnken响应面法优化提取工艺。最优条件提取后,利用HPLC法检测不同干燥方式肉苁蓉的松果菊苷、肉苁蓉苷A、毛蕊花糖苷、异毛蕊花糖苷、2′-乙酰基毛蕊花糖苷成分含量并采用单因素方差分析、聚类分析、主成分分析、密切值法分析5种苯乙醇苷类成分含量,选择最佳干燥方式。结果最佳提取工艺:甲醇体积分数55.14%,液料比46.39,提取时间38.50 min。聚类分析、主成分分析、密切值法分析表明,采用冷冻干燥法所得到的肉苁蓉品质最好,80℃烘干次之,40℃烘干最低。结论使用该工艺提取肉苁蓉,其5种苯乙醇苷类成分提取完全、充分。虽然冷冻干燥法炮制肉苁蓉所保留有效成分最高,但从生产角度考虑,采用80℃烘干法炮制能达到成本和功效的平衡。
|
| 关 键 词： | 肉苁蓉 提取工艺 Box-Behnken响应面法 干燥方式 HPLC 松果菊苷 肉苁蓉苷A 毛蕊花糖苷 异毛蕊花糖苷 2''-乙酰基毛蕊花糖苷 |
| 收稿时间： | 2019-04-17 |
Extraction parameters and drying methods of Cistanche deserticola |
| |
| HUANG Xiang,GUO Ye-hong,JIA Cun-qin,MA Xu-dong,YANG Li-ping,HUANG Guang-bin and KAN Jian-hong. Extraction parameters and drying methods of Cistanche deserticola[J]. Chinese Traditional and Herbal Drugs, 2019, 50(15): 3622-3630 |
| |
| Authors: | HUANG Xiang GUO Ye-hong JIA Cun-qin MA Xu-dong YANG Li-ping HUANG Guang-bin KAN Jian-hong |
| |
| Affiliation: | Gansu Key Laboratory of Arid Habitat Crop Science, Gansu Province Medicinal Plant Cultivation and Breeding Engineering Research Center, Key Laboratory of Standardized Production Technology Innovation of Traditional Chinese Medicine in Gansu Province, Chinese Herbal Medicine Research Institute, Agriculture College of Gansu Agricultural University, Lanzhou 730070, China,Gansu Key Laboratory of Arid Habitat Crop Science, Gansu Province Medicinal Plant Cultivation and Breeding Engineering Research Center, Key Laboratory of Standardized Production Technology Innovation of Traditional Chinese Medicine in Gansu Province, Chinese Herbal Medicine Research Institute, Agriculture College of Gansu Agricultural University, Lanzhou 730070, China,Gansu Huiqin Biological Technology Co., Ltd., Baiyin 730600, China,Gansu Key Laboratory of Arid Habitat Crop Science, Gansu Province Medicinal Plant Cultivation and Breeding Engineering Research Center, Key Laboratory of Standardized Production Technology Innovation of Traditional Chinese Medicine in Gansu Province, Chinese Herbal Medicine Research Institute, Agriculture College of Gansu Agricultural University, Lanzhou 730070, China,Gansu Key Laboratory of Arid Habitat Crop Science, Gansu Province Medicinal Plant Cultivation and Breeding Engineering Research Center, Key Laboratory of Standardized Production Technology Innovation of Traditional Chinese Medicine in Gansu Province, Chinese Herbal Medicine Research Institute, Agriculture College of Gansu Agricultural University, Lanzhou 730070, China,Gansu Key Laboratory of Arid Habitat Crop Science, Gansu Province Medicinal Plant Cultivation and Breeding Engineering Research Center, Key Laboratory of Standardized Production Technology Innovation of Traditional Chinese Medicine in Gansu Province, Chinese Herbal Medicine Research Institute, Agriculture College of Gansu Agricultural University, Lanzhou 730070, China and Gansu Key Laboratory of Arid Habitat Crop Science, Gansu Province Medicinal Plant Cultivation and Breeding Engineering Research Center, Key Laboratory of Standardized Production Technology Innovation of Traditional Chinese Medicine in Gansu Province, Chinese Herbal Medicine Research Institute, Agriculture College of Gansu Agricultural University, Lanzhou 730070, China |
| |
| Abstract: | |
| |
| Keywords: | Cistanche deserticola Y. C. Ma extraction process Box-Behnken response surface method drying method HPLC echinacoside cistanche A verbascoside isoacteoside 2''-acetylacteoside |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《中草药》浏览原始摘要信息 |
|
点击此处可从《中草药》下载全文 |
|
