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原花青素对慢性不可预知性应激小鼠脑内单胺递质的影响
引用本文:黄鹏,吴淑娟,叶涵,黄春燕,潘建春.原花青素对慢性不可预知性应激小鼠脑内单胺递质的影响[J].中国现代应用药学,2019,36(12):1473-1477.
作者姓名:黄鹏  吴淑娟  叶涵  黄春燕  潘建春
作者单位:温州医科大学附属第三医院, 浙江 瑞安 325200,温州医科大学附属第三医院, 浙江 瑞安 325200,温州医科大学药学院, 浙江 温州 325035,温州医科大学附属第三医院, 浙江 瑞安 325200,温州医科大学药学院, 浙江 温州 325035
基金项目:温州市科技计划项目(Y20170695)
摘    要:目的 研究原花青素在慢性不可预知性应激(unpredictable chronic mild stress,UCMS)小鼠中的抗抑郁样作用以及对小鼠脑内单胺递质含量的影响。方法 随机将ICR小鼠分为6组:空白对照组,UCMS组,原花青素低、中、高剂量组(UCMS+原花青素12.5,25,50 mg·kg-1),氟西汀组(UCMS+氟西汀10 mg·kg-1)。采用UCMS建立模型组评估小鼠的抑郁和焦虑样行为,采用HPLC测定小鼠海马、额叶皮层和下丘脑中单胺递质的含量。结果 与空白对照组比较,UCMS组小鼠悬尾的不动时间明显增加(P<0.05),大理石掩埋数目显著增加(P<0.01);小鼠海马、额叶皮层和下丘脑中的去甲肾上腺素、5-羟色胺和多巴胺的含量均减少;与UCMS组比较,原花青素组能明显逆转上述行为学以及脑内单胺递质含量的改变。结论 UCMS小鼠存在抑郁焦虑样行为学的改变,海马、额叶皮层和下丘脑中单胺递质含量表达异常。原花青素可以改善UCMS小鼠行为学的改变,并调节海马、额叶皮层和下丘脑中单胺递质的表达。

关 键 词:原花青素  抑郁  焦虑  慢性不可预知性应激  单胺递质
收稿时间:2018/8/14 0:00:00
修稿时间:2019/6/14 0:00:00

Effect of Proanthocyanidin on Monoamine Expression of Mice with Unpredictable Chronic Mild Stress
HUANG Peng,WU Shujuan,YE Han,HUANG Chunyan and PAN Jianchun.Effect of Proanthocyanidin on Monoamine Expression of Mice with Unpredictable Chronic Mild Stress[J].The Chinese Journal of Modern Applied Pharmacy,2019,36(12):1473-1477.
Authors:HUANG Peng  WU Shujuan  YE Han  HUANG Chunyan and PAN Jianchun
Institution:The Third Affiliated Hospital of Wenzhou Medical University, Rui''an 325200, China,The Third Affiliated Hospital of Wenzhou Medical University, Rui''an 325200, China,School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou 325035, China,The Third Affiliated Hospital of Wenzhou Medical University, Rui''an 325200, China and School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou 325035, China
Abstract:OBJECTIVE To explore the effect of proanthocyanidin on unpredictable chronic mild stress(UCMS) mice with depression-like behavior, and its effect on the content of monoamine. METHODS ICR mice were divided into 6 groups:blank group, UCMS group, proanthocyanidin low, medium, high dose groups(UCMS+proanthocyanidin 12.5, 25 and 50 mg·kg-1), fluoxetine group(UCMS+fluoxetine 10 mg·kg-1). The model of mice was established by UCMS, and then to evaluate depression-like behavior and anxiety-like behavior. The content of monoamine in the hippocampus, cortex and hypothalamus was determined by HPLC. RESULTS Compared with the control group, the duration of immobility of UCMS mice in the tail suspension test significantly increased(P<0.05), the number of the buried marble of UCMS mice increased(P<0.01). The expression of norepinephrine(NE), 5-hydroxytryptamine(5-HT) and dopamine(DA) decreased in the hippocampus, prefrontal cortex and hypothalamus of UCMS model group as compared with those in the control group. Proanthocyanidin improved the behavior of UCMS mice, and reversed the levels of 5-HT, NE and DA in the hippocampus, prefrontal cortex and hypothalamus. CONCLUSION UCMS model mice have abnormal change in the depression and anxiety-like behavior and expression of monoamine in the hippocampus, prefrontal cortex and hypothalamus. Proanthocyanidin can ameliorate the changes of the behavior and regulate the expression of monoamine in the hippocampus, prefrontal cortex and hypothalamus.
Keywords:proanthocyanidin  depression  anxiety  unpredictable chronic mild stress  monoamine
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