| 基于CDDP标记的重楼属种质资源鉴定及遗传多样性分析 |
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| 引用本文： | 周武先,段媛媛,卢超,由金文,何银生,王智,杨旭,尹晓蛟,程天周,安小玲,张美德.基于CDDP标记的重楼属种质资源鉴定及遗传多样性分析[J].中草药,2019,50(20):5033-5039. |
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| 作者姓名： | 周武先 段媛媛 卢超 由金文 何银生 王智 杨旭 尹晓蛟 程天周 安小玲 张美德 |
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| 作者单位： | 湖北省农业科学院中药材研究所 湖北省农业科技创新中心中药材分中心, 湖北 恩施 445000,湖北省农业科学院中药材研究所 湖北省农业科技创新中心中药材分中心, 湖北 恩施 445000,湖北省农业科学院中药材研究所 湖北省农业科技创新中心中药材分中心, 湖北 恩施 445000,湖北省农业科学院中药材研究所 湖北省农业科技创新中心中药材分中心, 湖北 恩施 445000,湖北省农业科学院中药材研究所 湖北省农业科技创新中心中药材分中心, 湖北 恩施 445000,湖南中医药大学药学院, 湖南 长沙 410208,湖北生态工程职业技术学院, 湖北 武汉 430200,湖北生态工程职业技术学院, 湖北 武汉 430200,恩施程丰农业综合开发有限公司, 湖北 恩施 445000,渭南市农业科学研究所, 陕西 渭南 714000,湖北省农业科学院中药材研究所 湖北省农业科技创新中心中药材分中心, 湖北 恩施 445000 |
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| 基金项目： | 湖北省中央引导地方科技发展专项资金（2018ZYYD013）；湖北省中央引导地方科技发展专项资金（2019ZYYD064）；恩施州科技计划研究与开发项目（D20180014）；湖北省农业科学院青年科学基金项目（2019NKYJJ13） |
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| 摘 要： | 目的研究重楼属植物遗传多样性,并为其种质资源的快速鉴定提供有效方法。方法采用CDDP分子标记方法对13份不同的重楼种质资源的遗传多样性进行分析,并按照CODE128条形码编码规则为重楼属植物进行编码。结果 11条引物共扩增了80个条带,其中多态性条带73个,多态性比率91.25%,等位基因数(Na)为1.912 5,有效等位基因数(Ne)为1.589 6,Nei’s遗传多样性指数(H)为0.342 3,Shannon信息指数(I)为0.507 0。UPGMA聚类分析表明,重楼属植物遗传多样性丰富。在CDDP分子标记的基础上依据CODE128条形码编码规则为重楼属植物构建了条形码分子身份证。结论重楼属资源遗传多样性丰富,CDDP分子标记可用于重楼属种质资源的鉴定及遗传多样性分析,且构建的条形码分子身份证检测灵敏、快捷,可用于相关科研工作和工业生产中重楼属植物的鉴定。
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| 关 键 词： | 重楼属 CDDP标记 种质资源 遗传多样性 条形码分子身份证 |
| 收稿时间： | 2019/5/4 0:00:00 |
Genetic diversity analysis and germplasm identification of Paris species by CDDP markers |
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| ZHOU Wu-xian,DUAN Yuan-yuan,LU Chao,YOU Jin-wen,HE Yin-sheng,WANG Zhi,YANG Xu,YIN Xiao-jiao,CHENG Tian-zhou,AN Xiao-ling and ZHANG Mei-de.Genetic diversity analysis and germplasm identification of Paris species by CDDP markers[J].Chinese Traditional and Herbal Drugs,2019,50(20):5033-5039. |
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| Authors: | ZHOU Wu-xian DUAN Yuan-yuan LU Chao YOU Jin-wen HE Yin-sheng WANG Zhi YANG Xu YIN Xiao-jiao CHENG Tian-zhou AN Xiao-ling and ZHANG Mei-de |
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| Institution: | Chinese Medicinal materials Branch of Hubei Agriculture Technology Innovation Center, Institute of Chinese Herbal Medicine of Hubei Academy of Agriculture Sciences, Enshi 445000, China,Chinese Medicinal materials Branch of Hubei Agriculture Technology Innovation Center, Institute of Chinese Herbal Medicine of Hubei Academy of Agriculture Sciences, Enshi 445000, China,Chinese Medicinal materials Branch of Hubei Agriculture Technology Innovation Center, Institute of Chinese Herbal Medicine of Hubei Academy of Agriculture Sciences, Enshi 445000, China,Chinese Medicinal materials Branch of Hubei Agriculture Technology Innovation Center, Institute of Chinese Herbal Medicine of Hubei Academy of Agriculture Sciences, Enshi 445000, China,Chinese Medicinal materials Branch of Hubei Agriculture Technology Innovation Center, Institute of Chinese Herbal Medicine of Hubei Academy of Agriculture Sciences, Enshi 445000, China,School of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China,Hubei Ecology Polytechnic College, Wuhan 430200, China,Hubei Ecology Polytechnic College, Wuhan 430200, China,Enshi Chengfeng Agriculture Co., Ltd., Enshi 445000, China,Weinan Institude of Agriculture Sciences, Weinan 714000, China and Chinese Medicinal materials Branch of Hubei Agriculture Technology Innovation Center, Institute of Chinese Herbal Medicine of Hubei Academy of Agriculture Sciences, Enshi 445000, China |
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| Abstract: | Objective To explore genetic diversity and phylogenic relationship among different types of Paris species and provide an effective method for the rapid identification of germplasm resources. Methods CDDP marker was used to evaluate the genetic diversity and phylogenic relationship among 13 types of Paris species. And the coding of Paris genus plants was carried out based on method of CODE128 barcode. Results Our results indicated that 73 polymorphic bands were amplified by 11 primers among 80 bands, and the ratio of polymorphic band was 91.25%. The observed number of alleles (Na), effective number of alleles (Ne), Nei''s gene diversity (H), and Shannon''s information index (I) was 1.912 5, 1.589 6, 0.342 3, and 0.507 0, respectively. The results of UPGMA analysis showed that there were great differences among 13 types of Paris species on genetic diversity. Based on CDDP markers, 13 barcode molecular identity cards were constructed for Paris species based on method of CODE128 barcode. Conclusion There was abundant polymorphism among Paris species, and CDDP markers were effective to analyze the genetic diversity of Paris species, and the established barcode molecular identity for Paris species was sensitive and fast, which can be used for scientific research and industrial production of Paris genus. |
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| Keywords: | Paris L CDDP marker germplasm resources genetic diversity barcode molecular identity |
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