| 基于网络药理学的气滞胃痛颗粒抗炎镇痛活性作用分析 |
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| 引用本文： | 杨晓娟,胡黄婉茵,张海珠,郝俊杰,刘靖,刘晓熠,王玄,邬国松,肖小河,牛明,罗光明.基于网络药理学的气滞胃痛颗粒抗炎镇痛活性作用分析[J].中草药,2019,50(13):3094-3106. |
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| 作者姓名： | 杨晓娟 胡黄婉茵 张海珠 郝俊杰 刘靖 刘晓熠 王玄 邬国松 肖小河 牛明 罗光明 |
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| 作者单位： | 江西中医药大学药学院, 江西 南昌 330004;中国人民解放军总医院第五医学中心, 全军中医药研究所, 北京 100039,中国人民解放军总医院第五医学中心, 全军中医药研究所, 北京 100039,大理大学药学与化学学院, 云南 大理 671000,中国人民解放军总医院第五医学中心, 全军中医药研究所, 北京 100039,中国人民解放军总医院第五医学中心, 全军中医药研究所, 北京 100039,中国人民解放军总医院第五医学中心, 全军中医药研究所, 北京 100039,中国人民解放军总医院第五医学中心, 全军中医药研究所, 北京 100039,中国人民解放军总医院第五医学中心, 全军中医药研究所, 北京 100039,中国人民解放军总医院第五医学中心, 全军中医药研究所, 北京 100039,中国人民解放军总医院第五医学中心, 全军中医药研究所, 北京 100039,江西中医药大学药学院, 江西 南昌 330004 |
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| 基金项目： | 国家中药标准化项目（ZYBZH-C-HUN-21）;国家中药标准化项目（ZYBZH-Y-TJ-43）;国家中药标准化项目（ZYBZH-Y-BJ-07）：全军医学科技“十二五”科研项目（BWS11J049）;全军医学科技青年培育项目（16QNP151）;北京市科技计划-京津冀协同创新推动（Z171100004517014） |
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| 摘 要： | 目的通过网络药理学的方法预测气滞胃痛颗粒抗炎镇痛主要活性成分的作用靶点,结合中医方解配伍理论对其多成分-多靶点-多通路的作用进行分析。方法基于TCMSP中药系统生物学分析数据库收集气滞胃痛颗粒中6味中药的主要化学成分,并借助LC-MS技术对所筛成分进行分析,通过TCMSP检索和Pharmmapper软件预测获取各成分主要的作用靶标,并通过DIP数据库,利用蛋白质相互作用信息建立药物靶标与炎症疼痛靶标的关联,构建药物-靶标-疾病网络,通过网络特征分析气滞胃痛颗粒抗炎镇痛的作用靶标,阐释其抗炎镇痛的主要作用机制。结果根据网络分析,共有44个炎症疼痛靶点与气滞胃痛颗粒密切相关,其中直接作用靶点有20个,主要是对环加氧酶-2(COX-2)和诱导型一氧化氮合酶(i NOS)等蛋白酶的作用,作用机制可能与调节肿瘤坏死因子(TNF)信号通路、NOD样受体(NLR)信号通路、血管内皮生长因子(VEGF)信号通路等与炎症疼痛密切相关的信号通路有关。结论气滞胃痛颗粒抗炎镇痛作用体现了中药多成分、多靶点、多途径的作用特点,该研究为深入阐释气滞胃痛颗粒抗炎镇痛作用机制提供科学依据,并且进一步说明了中医药古方配伍理论的科学性。
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| 关 键 词： | 气滞胃痛颗粒 抗炎镇痛 网络药理学 作用靶点 配伍理论 |
| 收稿时间： | 2018/12/19 0:00:00 |
Anti-inflammatory and analgesic activity analysis of Qizhi Weitong Granules based on network pharmacology |
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| YANG Xiao-juan,HU Huang Wan-yin,ZHANG Hai-zhu,HAO Jun-jie,LIU Jing,LIU Xiao-yi,WANG Xuan,WU Guo-song,XIAO Xiao-he,NIU Ming and LUO Guang-ming.Anti-inflammatory and analgesic activity analysis of Qizhi Weitong Granules based on network pharmacology[J].Chinese Traditional and Herbal Drugs,2019,50(13):3094-3106. |
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| Authors: | YANG Xiao-juan HU Huang Wan-yin ZHANG Hai-zhu HAO Jun-jie LIU Jing LIU Xiao-yi WANG Xuan WU Guo-song XIAO Xiao-he NIU Ming and LUO Guang-ming |
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| Institution: | School of Pharmacy, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China;Department of China Military Institute of Chinese Materia, the Fifth Medical Centre, Chinese PLA(People''s Liberation Army) General Hospital, Beijing 100039, China,Department of China Military Institute of Chinese Materia, the Fifth Medical Centre, Chinese PLA(People''s Liberation Army) General Hospital, Beijing 100039, China,Department of Pharmacy and Chemistry, Dali University, Dali 671000, China,Department of China Military Institute of Chinese Materia, the Fifth Medical Centre, Chinese PLA(People''s Liberation Army) General Hospital, Beijing 100039, China,Department of China Military Institute of Chinese Materia, the Fifth Medical Centre, Chinese PLA(People''s Liberation Army) General Hospital, Beijing 100039, China,Department of China Military Institute of Chinese Materia, the Fifth Medical Centre, Chinese PLA(People''s Liberation Army) General Hospital, Beijing 100039, China,Department of China Military Institute of Chinese Materia, the Fifth Medical Centre, Chinese PLA(People''s Liberation Army) General Hospital, Beijing 100039, China,Department of China Military Institute of Chinese Materia, the Fifth Medical Centre, Chinese PLA(People''s Liberation Army) General Hospital, Beijing 100039, China,Department of China Military Institute of Chinese Materia, the Fifth Medical Centre, Chinese PLA(People''s Liberation Army) General Hospital, Beijing 100039, China,Department of China Military Institute of Chinese Materia, the Fifth Medical Centre, Chinese PLA(People''s Liberation Army) General Hospital, Beijing 100039, China and School of Pharmacy, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China |
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| Abstract: | Objective The target of anti-inflammatory and analgesic active components of Qizhi Weitong Granules was predicted by network pharmacology method, and the effect of multi-component-multi-target-multi-pathways on TCM-complexation theory was analyzed. Methods The main chemical constituents of six Chinese medicines in Qizhi Weitong Granules were collected based on the TCMSP Chinese Medicine System Biological Analysis Database and analyzed by LC-MS. The main target of each component was predicted by TCMSP search and Pharmmapper software. The relationship between drug, target and inflammatory pain target was established by DIP database and protein interaction information. In order to elucidate the main mechanism of anti-inflammatory and analgesic effects of Qizhi Weitong Granules, the network of "drug-target-disease" was constructed and the target of anti-inflammatory and analgesic effects of Qizhi Weitong Granules were analyzed by network characteristics. Results Through the network analysis, a total of 44 inflammatory targets were closely related to Qizhi Weitong Granules, of which 20 were directly targeted, mainly for the action of proteases such as COX-2 and iNOS. The mechanism may be related to the regulation of TNF signaling pathway, NOD-like receptor signaling pathways, VEGF signaling pathways and other signaling pathways closely related to inflammation. Conclusion The anti-inflammatory and analgesic effects of Qizhi Weitong Granules reflect the multi-component, multi-target and multi-pathway characteristics of traditional Chinese medicine. This study provides a scientific basis for further understanding the anti-inflammatory and analgesic mechanism of Qizhi Weitong Granules and explains the scientificity of the traditional Chinese medicine compatibility theory. |
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| Keywords: | Qizhi Weitong Granules anti-inflammatory and analgesic activity network pharmacology target compatibility theory |
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