| 精氨酸双糖苷检测方法的建立及药动学研究 |
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| 引用本文： | 毕筱婷,王长远,李珂珂,霍晓奎,刘克辛,弓晓杰.精氨酸双糖苷检测方法的建立及药动学研究[J].现代药物与临床,2019,42(6):1141-1145. |
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| 作者姓名： | 毕筱婷 王长远 李珂珂 霍晓奎 刘克辛 弓晓杰 |
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| 作者单位： | 大连大学 医学院, 辽宁 大连 116622,大连医科大学 药学院临床药理教研室, 辽宁 大连 116044,大连大学 医学院, 辽宁 大连 116622,大连医科大学 药学院临床药理教研室, 辽宁 大连 116044,大连医科大学 药学院临床药理教研室, 辽宁 大连 116044,大连民族大学 生命科学院, 辽宁 大连 116600 |
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| 基金项目： | 辽宁省高等学校创新团队支持计划（LT2016017）；大连市科技创新基金（2018J12SN062） |
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| 摘 要： | 目的 建立快速、灵敏、高效的精氨酸双糖苷的测定方法并研究精氨酸双糖苷在大鼠体内的药动学。方法 以乌苯美司作为内标，采用LC-MS/MS检测方法测定血浆中精氨酸双糖苷浓度，利用DAS2.0软件计算药动学相关参数，并分析方法的可靠性。结果 空白血浆对检测物质不产生干扰，在20~20 000 ng/mL符合线性关系，方法学中RSD以及RE值均小于15%。结论 采用LC-MS/MS方法检测精氨酸双糖苷方法合理可靠，精氨酸双糖苷的绝对生物利用度为（4.50±3.75）%。
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| 关 键 词： | 精氨酸双糖苷 精密度 准确度 药动学 方法学 |
| 收稿时间： | 2019/2/14 0:00:00 |
Establishment of a method for detecting Arginyl-fructosyl-glucose and pharmacokinetic study |
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| BI Xiaoting,WANG Changyuan,LI keke,HUO Xiaokui,LIU Kexin and GONG Xiaojie.Establishment of a method for detecting Arginyl-fructosyl-glucose and pharmacokinetic study[J].Drugs & Clinic,2019,42(6):1141-1145. |
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| Authors: | BI Xiaoting WANG Changyuan LI keke HUO Xiaokui LIU Kexin and GONG Xiaojie |
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| Institution: | Medical college of Dalian University, Dalian 116622, China,Department of Clinical Pharmacology, College of Pharmacy, Dalian Medical University, Dalian 116044, China,Medical college of Dalian University, Dalian 116622, China,Department of Clinical Pharmacology, College of Pharmacy, Dalian Medical University, Dalian 116044, China,Department of Clinical Pharmacology, College of Pharmacy, Dalian Medical University, Dalian 116044, China and College of Life Science, Dalian Nationalities University, Dalian 116600, China |
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| Abstract: | Objective To develop a quick, specific and high performance method for determination of Arginyl-fructosyl-glucose (AFG) and to investigate pharmacokinetics of AFG in rats in vivo. Methods Bestatin is used as an internal standard, the concentration of AFG in plasma was determined by LC-MS/MS. The DAS 2.0 software was adopted to calculate bioavailability of AFG. Results Blank plasma does not interfere with the test substance, there is a significant correlation in the range of 20 ng/mL to 20 000 ng/mL, and the RSD and RE values are less than 15% in the method. Conclusion The average relative bioavailability of AFG was (4.50 ±3.75)%, The measured method of AFG by LC-MS/MS is reasonable and reliable. |
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| Keywords: | Arginyl-fructosyl-glucose relative standard deviation relative error pharmacokinetic methodology |
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