人参多糖对一次性力竭游泳大鼠红细胞功能恢复的影响

目的探讨人参多糖对一次性力竭游泳模型大鼠红细胞功能恢复的作用。方法采用大鼠力竭游泳模型,分为对照组、模型组、力竭后自恢复(自恢复)组及人参多糖低、中、高剂量组,采用ELISA方法对大鼠红细胞氧化损伤程度、大鼠血清中ATP、ATPase、唾液酸(SA)含量以及大鼠红细胞Na+, K+-ATPase、Ca~(2+), Mg~(2+)-ATPase活力进行检测,采用Western blotting方法检测红细胞膜Band3蛋白的水平,并测定由Band3蛋白变化引起的糖酵解关键酶活性的变化。结果力竭游泳后大鼠红细胞受到氧化应激损伤,红细胞抗氧化能力较对照组有明显下降,人参多糖能促进红细胞抗氧化能力恢复,且呈剂量依赖性;力竭游泳后大鼠血清中ATP、ATPase、SA含量较对照组相比下降,随着人参多糖剂量的增加,ATP、ATPase、SA含量逐渐回升;力竭游泳后红细胞Na+, K+-ATPase、Ca~(2+), Mg~(2+)-ATPase活力均有所下降,人参多糖可使Na+, K+-ATPase、Ca~(2+), Mg~(2+)-ATPase活力得到恢复,且呈剂量依赖性;力竭游泳后Band 3蛋白含量较对照组有明显下降,人参多糖可恢复红细胞膜Band3蛋白含量及其调节糖酵解关键酶丙酮酸激酶(PK)、己糖激酶(HK)、果糖-6-磷酸激酶(PFK)的活力,且呈剂量依赖性。结论人参多糖对力竭运动造成的大鼠红细胞功能损伤有较好的恢复作用。

Study on recovery of erythrocyte function in one-time exhaustive swimming rats by ginseng polysaccharide

Objective To investigate the effect of ginseng polysaccharide on the recovery of erythrocyte function in rats with one-time exhaustive swimming. Methods Rat exhaustion swimming model was divided into control group, exhaustion group, self-recovery group after exhaustion, and low, middle and high dose groups of ginseng polysaccharide. The degree of oxidative damage of erythrocyte, ATP, ATPase, SA content in rat serum were detected by ELISA, and the activity of Na⁺, K⁺-ATPase and Ca²⁺, Mg²⁺-ATPase in rat red blood cells were detected. The level of Band 3 protein in erythrocyte membrane was detected by Western blot and changes in the activities of key enzymes of glycolysis caused by Band 3 protein were determined. Results After exhaustive swimming, the red blood cells of rats were damaged by oxidative stress. The anti-oxidant capacity of red blood cells was significantly lower than that of the control group. The ginseng polysaccharide could promote the recovery of anti-oxidant capacity of red blood cells in a dose-dependent manner. The content of ATPase and SA were decreased compared with the control group. With the increase of ginseng polysaccharide concentration, the content of ATP, ATPase and SA were increased gradually. The activities of Na⁺, K⁺-ATPase and Ca²⁺, Mg²⁺-ATPase were decreased after exhaustive swimming. The ginseng polysaccharide can restore the function of sodium potassium pump and calcium magnesium pump in a dose-dependent manner. The content of Band 3 protein after exhaustive swimming was significantly lower than that of the blank control group. The ginseng polysaccharide can restore the content of erythrocyte membrane Band 3 protein. The activities of the key enzymes of glycolysis, PK, HK and PFK, were adjusted in a dose-dependent manner. Conclusion Ginseng polysaccharide has a good recovery effect on rat erythrocyte function damage caused by exhaustive exercise.