不同海拔和光照对黄芩中7种黄酮类有效成分含量的影响

目的分析不同海拔、光照因素对黄芩中7种黄酮类有效成分量的影响,为黄芩种植最佳生长条件的选择提供理论依据。方法采用HPLC法同时测定人工种植的24批黄芩样品中野黄芩苷、黄芩苷、汉黄芩苷、芹菜素、黄芩素、汉黄芩素、千层纸素A7种成分的含量,并采用方差分析法进行不同海拔、光照环境(阴坡、阳坡)与各成分量的相关性分析。结果同一生长年限,在海拔(550±30)、(650±30)m 2个范围,对黄芩中7种成分的量进行比较,其中黄芩苷、汉黄芩苷有极显著性差异(P0.01),野黄芩苷、黄芩素、千层纸素A、汉黄芩素、芹菜素有显著性差异(P0.05);阴坡和阳坡2种不同的光照条件下,黄芩中7种成分的量相比,无显著性差异(P0.05)。结论在同一生长年限,随海拔的升高,黄芩中黄酮类成分的量都呈现明显的上升趋势,有显著性差异。而光照(阴坡、阳坡)不同时,黄芩中黄酮类成分的量无显著性差异,但除汉黄芩苷、黄芩素外,其他黄酮类成分含量的均数阳坡高于阴坡。黄芩人工种植宜选择阳光直接照射的平地或阳坡,同时及海拔偏高有利于提高黄芩中黄酮类化学成分总量的积累。

Effects of different elevations and illumination on content of seven active components in Scutellaria baicalensis

Objective Effects of different elevations and illumination on the seven effective components of flavonoids in Scutellaria baicalensis were analyzed, providing a theoretical basis for selecting the best growing conditions. Methods The content of cutellarin, baicalin, wogonoside, apigenin, baicalein, wogonin, and oroxylin A were determined by HPLC method in 24 artificially planted samples. The correlation between different elevations, different lighting conditions (shady slope, sunny slope) and different components were analyzed by using variance analysis method. Results Compared with the same growth period, the amount of seven components in S. baicalensis was compared at elevation (550 ±30) and (650 ±30) m, and there was a significant difference between baicalins and wogonoside (P < 0.01). There have significant differences in the content of cutellarin, apigenin, baicalein, wogonin, oroxylin A, and apigenin (P < 0.05); There was no significant difference in the amount of seven components in S. baicalensis under different illumination conditions (P > 0.05). Conclusion In the same growing age, the amount of flavonoids in S. baicalensis showed an obvious upward trend with the increase of altitude, and there were significant differences. There was no significant difference in the content of flavonoids in S. baicalensis, but the average sunny slope of various components of other flavonoids was higher than the shady slope except for wogonoside and baicalein. Therefore, it is advisable to choose the plain or sunny slope directly illuminated by sunlight in the artificial cultivation of S. baicalensis, and the high altitude is conducive to increasing the accumulation of total flavonoids in S. baicalensis.