他克莫司血药浓度检测方法的研究进展

他克莫司是一种大环内酯类免疫抑制剂，临床上广泛用于器官移植和自身免疫系统疾病的治疗，其疗效和不良反应与该药血药浓度密切相关，并且个体差异性较大。临床上常用液相色谱串联质谱法（LC-MS/MS）、微粒子酶免疫分析法（MEIA）、酶联免疫吸附法（ELISA）、酶放大免疫分析法（EMIT）、化学发光微粒子免疫法（CMIA）等检测技术对他克莫司血药浓度进行检测。对他克莫司血药浓度检测方法的研究进展进行综述，为临床合理用药和相关领域的研究提供借鉴。

Research progress on detection methods of tacrolimus blood concentration

Tacrolimus is a macrolide immunosuppressive agent widely used in the treatment of organ transplantation and autoimmune diseases. The efficacy and adverse reactions are closely related to the blood drug concentration of the drug, and the individual differences are large. Clinical monitoring of tacrolimus blood concentrations is usually performed by performance liquid chromatography-mass spectrometry (LC-MS/MS), microparticle enzyme immunoassay assay (MEIA), enzyme-linked immunosorbent assay (ELISA), enzyme-multipliedimmuno assay technique (EMIT), and chemiluminescence microparticle immunoassay assay (CMIA), etc. The research progress on detection methods of tacrolimus blood concentration is reviewed in this paper, which provides reference for the clinical rational drug use and related research in this field.