基于超滤-络合萃取技术的甘草酸制备工艺研究

目的建立一种采用超滤-络合萃取技术制备甘草酸的工艺。方法考察pH值(p H 4～8)对超滤液中甘草酸萃取率的影响;采用正交试验优选络合萃取甘草酸的工艺条件;通过考察反萃取剂种类及浓度,确定甘草酸的反萃取工艺条件。结果所得甘草酸最佳络合萃取工艺条件为超滤液pH值应调至2、萃取剂为三烷基氧化膦(TRPO)-磺化煤油(5∶95)、有机相与水相体积比为1∶1,甘草酸平均萃取率达到99.2%。甘草酸最佳反萃取工艺条件为22.5 mmol/L NaOH水溶液为反萃取剂、有机相与反萃取剂体积比1∶1,甘草酸单次反萃取率达到98.8%,甘草酸总转移率为98.1%。结论超滤-络合萃取技术可作为甘草酸制备的一种新工艺。

Preparation technology of glycyrrhizin acid based on ultrafiltration-complexation extraction technology

Objective To establish a technique for preparing glycyrrhizin acid (GA) by ultrafiltration-complexation extraction. Methods The effect of pH on the extraction rate of GA from ultrafiltrate was investigated in the range of 4-8; The orthogonal test was used to optimize the technological conditions for complexation extraction of GA; The extraction conditions of GA were determined by investigating the type and concentration of the extractant. Results The optimum extraction conditions for GA were as follows:pH value of Glycyrrhiza uralensis ultrafiltrate was adjusted to 2, TRPO-sulfonated kerosene (5:95, volume percent), volume ratio of organic phase to aqueous phase was 1:1, and average extraction rate of GA reached 99.2%. The best back extraction conditions for GA were as follows:22.5 mmol/L NaOH aqueous solution was used as the stripping agent, the volume ratio of organic phase to stripping agent was 1:1, the single back extraction rate of GA reached 98.8%, and the total transfer rate of GA was 98.1%. Conclusion Ultrafiltration-complexation extraction technology can be used as a new process for the preparation of GA.