血竭提取物对急性心衰大鼠脑缺血耐受的作用研究

目的 研究血竭提取物对大鼠急性心力衰竭（acute heart failure，AHF）致脑功能损伤的保护作用及其机制。方法 将25只SD大鼠随机分为空白对照组、心衰模型组和血竭提取物干预组。空白对照组和心衰模型组每日生理盐水灌胃，干预组按照血竭提取物浓度分为低剂量（300 mg·kg^-1·d^-1）、中剂量（450 mg·kg^-1·d^-1）和高剂量（600 mg·kg^-1·d^-1）3组，每日灌胃。采用Morris水迷宫检测大鼠的空间学习记忆能力的变化；检测超氧化物歧化酶（superoxide dismutase，SOD）活性及丙二醛（malondialdehyde，MDA）含量；HE染色观察脑组织病理变化。结果 Morris水迷宫试验中，空白对照组和血竭提取物高、中剂量组的逃避潜伏期均短于心衰模型组，运动平均速度和目标象限时间百分比均大于心衰模型组；心衰模型组的MDA含量较空白对照组增加，SOD活性反之；血竭提取物干预组的MDA含量较心衰模型组下降，SOD活性反之。结论 急性心力衰竭单次发作即可损伤脑功能，血竭提取物可能通过抗氧化和清除氧自由基等对防治大鼠急性心力衰竭所致的脑功能损伤具有积极的保护作用。

Effect of Dragon's Blood Extract on Cerebral Ischemic Tolerance in Rats with Acute Heart Failure

OBJECTIVE To study the protective effect and mechanism of dragon''s blood extract on brain function damage induced by acute heart failure(AHF) in rats. METHODS Twenty-five SD rats were randomly divided into blank control group, heart failure model group and dragon''s blood extract intervention group. The blank control group and the heart failure model group rats were given normal saline daily by intragastric administration every day. The dragon''s blood extract intervention group was divided into three groups according to the concentration of dragon''s blood extract, the intervention group rats were given daily dragon''s blood extract 300 mg·kg^-1·d^-1 in the low dose group, 450 mg·kg^-1·d^-1 in the medium dose group and 600 mg·kg^-1·d^-1 in the high dose group by intragastric administration respectively. The Morris water maze was used to detect the changes of spatial learning and memory ability of rats. The contents of superoxide dismutase(SOD) and malondialdehyde(MDA) were detected. HE staining were used to observe brain tissue pathological changes. RESULTS In the Morris water maze experiment, the escape latency of the blank control group and the high and medium dose groups of dragon''s blood extract were shorter than that of the heart failure model group, and the average moving speed and target quadrant time percentage were greater than the heart failure model group. The content of MDA in the heart failure model group was increased compared with the blank control group, and the activity of SOD was opposite. The content of MDA in the dragon''s blood extract intervention group was lower than that of the heart failure model group, and the activity of SOD was opposite. CONCLUSION A single episode of AHF can impair brain function. Dragon''s blood extract may have a positive protective effect on the prevention and treatment of brain damage caused by AHF in rats through anti-oxidation and scavenging oxygen free radicals.