| 低场-高场核磁联用分析缔合态对平肺方中芒果苷提取分离的影响 |
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| 引用本文： | 吴鑫,左雯雯,李存玉,顾佳美,彭国平.低场-高场核磁联用分析缔合态对平肺方中芒果苷提取分离的影响[J].中草药,2019,50(6):1316-1322. |
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| 作者姓名： | 吴鑫 左雯雯 李存玉 顾佳美 彭国平 |
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| 作者单位： | 南京中医药大学药学院, 江苏 南京 210023,南京中医药大学药学院, 江苏 南京 210023,南京中医药大学药学院, 江苏 南京 210023;江苏省中药资源产业化过程协同创新中心, 江苏 南京 210023,南京中医药大学药学院, 江苏 南京 210023,南京中医药大学药学院, 江苏 南京 210023;江苏省中药资源产业化过程协同创新中心, 江苏 南京 210023 |
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| 基金项目： | 国家自然科学基金（81503258）；国家自然科学基金（81373980）；国家自然科学基金（81603307）；江苏省高校自然科学基金（17KJB360010）；中药标准化项目“新生化颗粒的标准化建设”（ZYBZH-C-JS-34）；江苏省自然科学基金青年基金（BK20151005） |
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| 摘 要： | 目的采用低场-高场核磁联用技术探索缔合态对平肺方中芒果苷提取、分离行为的影响。方法以1-脱氧野尻毒霉素(DNJ)-柠檬酸成分为对照,以氢T_2弛豫时间及氢化学位移值的变化为指标,采用低场核磁分析缔合态氢的弛豫特征,结合高场核磁技术验证缔合物结构。结果 DNJ-芒果苷在溶液中以缔合态的形式存在,相对分子质量增大,从而抑制了成分从药材向溶液的迁移,透过率下降,图谱中T_2峰向左偏移,高场核磁中化学位移也发生变化。结论阐明了成分存在状态对芒果苷提取分离行为的影响机制,为研究中药复方煎煮方法及机制探索提供了技术支撑。
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| 关 键 词： | 复方提取 平肺方 芒果苷 缔合物 核磁共振技术 T2弛豫时间 |
| 收稿时间： | 2018/6/20 0:00:00 |
Effects of association state on decocting rate of mangiferin in Pingfei Decoction by using low-field and high-field NMR |
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| WU Xin,ZUO Wen-wen,LI Cun-yu,GU Jia-mei and PENG Guo-ping.Effects of association state on decocting rate of mangiferin in Pingfei Decoction by using low-field and high-field NMR[J].Chinese Traditional and Herbal Drugs,2019,50(6):1316-1322. |
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| Authors: | WU Xin ZUO Wen-wen LI Cun-yu GU Jia-mei and PENG Guo-ping |
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| Institution: | School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China,School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China,School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China;Jiangsu Collaborative Innovation Center of Chinese Medicine Resources Industrialization, Nanjing 210023, China,School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China and School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China;Jiangsu Collaborative Innovation Center of Chinese Medicine Resources Industrialization, Nanjing 210023, China |
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| Abstract: | Objective To study the effects of association complexes on extraction and separation behavior of mangiferin in Pingfei Decoction by low-field and high-field NMR. Methods Using the T2 relaxation time and chemical shifts value of hydrogen as index, and group DNJ-citric acid as control, the relaxation characteristrics of hydrogen in association state was analyzed by low field NMR to verify the structure of association complexes combined with high-field NMR. Results DNJ and mangiferin existed in the form of association state with larger molecular weight, which inhibited the migration of components from medicinal materials to solution and caused lower transmittance. And the T2 peak shifted to the left in the spectrum, and the chemical shift in the high field nuclear magnetic field also changed. Conclusion This experiment clarified the mechanism of the effect of presence of components on extraction and separation behavior of mangiferin and provided technical support for studying the decocting method and mechanism of Chinese herbal compound. |
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| Keywords: | extraction of compound Chinese herbal medicine Pingfei Decoction mangiferin association complex nuclear magnetic technology T2 relaxation time |
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