| 基于层次分析法结合Box-Behnken设计-响应面法优选养阴清肺汤加味提取工艺 |
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| 引用本文： | 吴振起,高畅,杨璐,于艳,齐兆东. 基于层次分析法结合Box-Behnken设计-响应面法优选养阴清肺汤加味提取工艺[J]. 中草药, 2019, 50(12): 2862-2867 |
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| 作者姓名： | 吴振起 高畅 杨璐 于艳 齐兆东 |
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| 作者单位： | 辽宁中医药大学附属医院, 辽宁 沈阳 110032,辽宁中医药大学研究生学院, 辽宁 沈阳 110847,辽宁中医药大学研究生学院, 辽宁 沈阳 110847,辽宁中医药大学附属医院, 辽宁 沈阳 110032,辽宁中医药大学研究生学院, 辽宁 沈阳 110847 |
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| 基金项目： | 国家自然科学基金面上项目（81373687）；国家自然科学基金面上项目（81874490）；第四批全国中医（临床、基础）优秀人才研修项目（国中医药人教发[2017]24号）；辽宁省“百千万人才工程”人选资助项目（辽人社[2018]47号）；沈阳市中青年科技创新人才支持项目（RC180246） |
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| 摘 要： | 目的 优选养阴清肺汤加味提取工艺。方法 采用HPLC法同时测定毛蕊花糖苷、丹皮酚、绿原酸、甘草酸，以层次分析法（AHP）计算权重系数，多指标综合评分结合Box-Behnken设计-响应面法考察乙醇体积分数、提取时间、提取次数对养阴清肺汤加味提取工艺的影响，优选醇提工艺。结果 最佳提取工艺为采用69%乙醇，提取68 min，提取1次。结论 优选的提取工艺简单可行，成分提取效率高，为养阴清肺汤加味提取工艺提供参考。
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| 关 键 词： | 养阴清肺汤加味 Box-Behnken设计-响应面法 AHP法 提取工艺 毛蕊花糖苷 丹皮酚 绿原酸 甘草酸 HPLC |
| 收稿时间： | 2019-02-17 |
Optimization of extraction process of Modified Yangyin Qingfei Decoction by Box-Behnken response surface methodology based on analytic hierarchy process |
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| WU Zhen-qi,GAO Chang,YANG Lu,YU Yan and QI Zhao-dong. Optimization of extraction process of Modified Yangyin Qingfei Decoction by Box-Behnken response surface methodology based on analytic hierarchy process[J]. Chinese Traditional and Herbal Drugs, 2019, 50(12): 2862-2867 |
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| Authors: | WU Zhen-qi GAO Chang YANG Lu YU Yan QI Zhao-dong |
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| Affiliation: | Affiliated Hospital of Liaoning University of Traditional Chinese Medicine, Shenyang 110032, China,Graduate School of Liaoning University of Traditional Chinese Medicine, Shenyang 110847, China,Graduate School of Liaoning University of Traditional Chinese Medicine, Shenyang 110847, China,Affiliated Hospital of Liaoning University of Traditional Chinese Medicine, Shenyang 110032, China and Graduate School of Liaoning University of Traditional Chinese Medicine, Shenyang 110847, China |
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| Abstract: | Objective To optimize the extraction process of Modified Yangyin Qingfei Decoction (MYQD). Methods The effects of ethanol concentration, extraction time and extraction times on the optimization of extraction process of MYQD were investigated by multiple indicators comprehensive scoring and Box-Behnken response surface methodology. The content of verbascoside, chlorogenic acid, paeonol, and glycyrrhizic acid was simultaneously determined by HPLC, and the method of analytic hierarchy process was used to calculate the weight coefficient. Results The optimum extraction process was as follow:using 69% ethanol for once extraction for 68 min. Conclusion The optimum extraction process is simple and feasible, and the extraction efficiency of components is high, which can provide reference for the extraction process of MYQD. |
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| Keywords: | Modified Yangyin Qingfei Decoction Box-Behnken response surface methodology analytic hierarchy process extraction process verbascoside paeonol chlorogenic acid glycyrrhizic acid HPLC |
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