人群锰接触水平的早期生物标志物探索

目的 通过研究工人锰接触水平与生物材料中锰浓度的关系,探索人群接触锰的早期生物标志物.方法 用个体空气采样器采集空气锰,确定时间加权平均暴露剂量,按照不同接触剂量把工人分为对照组、低锰接触组、高锰接触组,测定各组工人发、尿、血清、血细胞及唾液中锰浓度,分析锰接触剂量与生物材料中锰浓度的关系和工龄与生物材料中锰浓度的关系.结果 高锰接触组唾液锰为32.17μg/L,发锰为37.39 mg/kg,尿锰为2.50 μg/L,血清锰为29.61 μg/,血细胞锰为14.49μg/L,均明显高于对照组(分别为10.40μg/L、1.60 mg/kg、0.77μg/L、10.30 μg/L、4.56 μg/L),差异均有统计学意义(P<0.01).唾液锰水平与工人当前锰接触剂量呈明显的正相关(r=0.649,P<0.01),与工人接触锰的工龄呈明显的正相关(r=0.404,P<0.01),与工人锰总接触量之间呈明显的正相关(r=0.342,P<0.01);唾液锰水平与血细胞锰、血清锰水平呈明显的正相关(r值分别为0.359,0.303,均P<0.01).结论 唾液锰可作为人群锰当前接触和历史接触的生物标志物.

Early biological markers of manganese exposure

Objective To explore the biomarker of manganese exposure by analyzing the relationship between manganese exposure and concentration in some biomaterials. Methods The air samples were collected through the individual air sample. According to the manganese levels in the air, workers were assigned to control group, low concentration group and high concentration group, and manganese in the hair, urine,serum, blood cell and saliva from different group were measured respectively. The correlations between concentration of external manganese exposure and manganese concentrations in biomaterials, and years of employment and concentrations in biomaterials were analyzed. Results In the high concentration group, saliva manganese was 32.17 μg/L, hair manganese was 37.39 mg/kg, urine manganese was 2.50 μg/L, plasma manganese was 29.61 μg/L, blood manganese was 14.49 μg/L, were higher than those in the control group( 10.40μg/L, 1.60 mg/kg, 0.77 μg/L, 10.30 μg/L, 4.56 μg/L respectively )( P<0.01 ). The manganese concentration in the saliva was significantly correlated with airborne manganese concentration (r=0.649, P<0.01 ), with the years of employment (r=0.404, P<0.01 ), with the total exposure of manganese (r=0.342, P<0.01 ), with the manganese concentration of plasma (r=0.303, P<0.01) and with the manganese concentration in blood cells (r=0.359, P<0.01 ), respectively. Conclusions The concentration of manganese in saliva could work as a biomarker of manganese internal exposure.