胃癌细胞异位分泌人绒膜促性腺激素对微血管密度的影响

目的研究胃癌组织中人绒膜促性腺激素（ＨＣＧ）对微血管密度（ＭＶＤ）的影响。方法运用ＳＰ法,对４０例胃癌手术切除标本进行抗人β促绒膜性腺激素（βＨＣＧ）多克隆抗体和抗因子ＦⅧ相关抗原抗体（ＦⅧＲＡｇ）免疫组织化学染色,观察癌组织、癌旁、正常组织各区域βＨＣＧ的表达和ＭＶＤ。结果所有胃癌组织中,βＨＣＧ仅在癌细胞内阳性表达,表达率为３７．５％（１５／４０）。ＭＶＤ在癌组织为２７．４±７．１,癌旁为１１．９±５．４,正常组织为４．８±１．５（Ｐ＜０．０５～０．０１）。βＨＣＧ阳性组的ＭＶＤ明显高于βＨＣＧ阴性组（Ｐ＜０．０５）,βＨＣＧ,ＭＶＤ和组织类型无关（Ｐ＞０．０５）,但βＨＣＧ和淋巴结转移有关（Ｐ＜０．０５）,伴有血道转移组的βＨＣＧ,ＭＶＤ均高于不伴血道转移组（Ｐ＜０．０５）。结论微血管生长与肿瘤的生长,血道转移有关,而ＨＣＧ对肿瘤的微血管有一定的促生长作用。

The effect of ectopic HCG on microvessel density in gastric carcinoma

OBJECTIVES: To study the effect of ectopic HCG on microvessel density in gastric carcinoma using immunohistochemical staining with anti-beta-HCG polyclonal antibody and anti-factor VIII related antigen(FVIII RAg)antibody. METHODS: A total of 40 specimens resected from patients with gastric carcinoma was investigated by immunohistochemical staining with a anti-beta-HCG polyclonal antibody and a monoclonal antibody against FVIII RAg. The expression of beta-HCG and the microvessel density(the average number of microvessel in three areas of highest vascular density at 400 x magnification) in various regions of the histologic specimens were studied. RESULTS: Fifteen (37.5%) of the 40 gastric carcinomas was positive for beta-HCG protein in tumor tissue. beta-HCG protein was negative in the tumor-adjacent and in normal tissue. The mean microvessel density was 27.4 +/- 7.1 in the tumor, 11.9 +/- 5.4 in tumor-adjacent and 4.8 +/- 1.5 in normal tissues (P < 0.05-0.01). There was a close correlation with beta-HCG positivity and microvessel density (MVD). There was no correlation between the degree of tumor cell differentiation and beta-HCG expression, nor was a correlation between it and MVD. High expression level of beta-HCG and MVD was significantly associated with hematogenous metastases. CONCLUSION: Ectopic HCG expression may have angiogenic effect which, in turn, may facilitate hematogenous dissemination of gastric cancer.