Northern印迹杂交分析nm23基因在人肺癌中的表达研究

目的探讨ｎｍ２３基因表达在人肺癌中的作用。方法通过Ｎｏｒｔｈｅｒｎ印迹杂交,检测４０例人肺癌组织和１９例非癌肺组织的ｎｍ２３Ｈ１和ｎｍ２３Ｈ２ｍＲＮＡ表达,采用地高辛标记和检测系统显示杂交信号,并分析ｍＲＮＡ表达与肺癌临床特征的关系。结果低分化鳞癌的ｎｍ２３Ｈ２ｍＲＮＡ表达较中高分化鳞癌显著降低（Ｐ＜０．０１）,小细胞肺癌的ｎｍ２３Ｈ１和ｎｍ２３Ｈ２ｍＲＮＡ表达较肺鳞癌明显降低。但在有或无淋巴结转移的原发癌灶组织间,以及肺癌的临床分期间,ｎｍ２３基因ｍＲＮＡ表达差异无显著性（Ｐ＞０．０５）。结论ｎｍ２３基因ｍＲＮＡ表达与肺癌的组织分化有关,未发现其在肺癌中的癌转移抑制作用。

Northern blot analysis of nm23 gene expression in human lung cancer

Objective To investigate the role of nm23 gene expression in lung cancer. Methods Forty human lung cancer tissues and 19 non cancerous pulmonary tissues were studied for their nm23 H1 and nm23 H2 mRNA expression with nonradioactive Northern blot hybridization. Correlation of nm23 mRNA expression with clinical features of lung cancer was analyzed. Results The mRNA expression of nm23 H2 gene in poorly differentiated squamous cell carcinoma was significantly decreased compared to that in moderately differentiated squamous cell carcinoma. The mRNA expression of nm23 H1 and nm23 H2 genes in small cell lung cancer was decreased compared to that in squamous cell carcinoma. No significant difference in nm23 mRNA expression was observed in lung cancers with and without lymph node metastasis, nor was there significant difference in patients with lung cancers in different stages. Conclusion The mRNA expression of nm23 gene is correlated with the degree of differentiation of lung cancer, but there is no evidence of metastasis suppression effect by nm23 gene.