肝硬化患者血清NO和NOS浓度分析及临床意义

目的 :探讨肝硬化 (LC)患者血清一氧化氮 (NO)、一氧化氮合酶 (NOS)浓度变化与肝功能分级及LC并发症间的关系。方法 :分别采用硝酸还原酶法和重叠比色法 ,检测 5 2例LC患者及 30例正常人血清NO和NOS浓度 ,并按患者的肝功能分级、是否伴 无并发症进行比较分析。结果 :LC患者血清NO水平和NOS浓度平均为 12 7.6 7μmol L和 4.2 5U ml,均非常明显地高于正常组 (P <0 .0 0 1) ;肝功能Child -Pugh分级显示 :血清NO水平和NOS浓度在C级显著地高于B级 (P <0 .0 5 )和A级 (P <0 .0 1) ;LC并发食管胃底静脉曲张破裂出血、自发性腹膜炎、肝病性低氧血症患者中血清NO水平和NOS浓度 ,均高于无并发症的LC患者 (P <0 .0 5 )。结论 :本文提示LC患者肝组织中NO和NOS合成增加 ,并与病情严重程度及患者有 无并发症密切相关

ANALYSIS AND CLINICAL SIGNIFICANCE OF SERUM NITRIC OXIDE AND NITRIC OXIDE SYNTHETASE LEVELS IN PATIENTS WITH LIVER CIRRHOSIS

Objective:To investigate the relationship between levels of serum nitric oxide (NO) or nitric oxide synthetase (NOS) and grades of liver function injury or complications of cirrhosis in patients with liver cirrhosis.Methods:The Levels of NO and NOS were analyzed in sera of 52 patients with liver cirrhosis(LC) and 30 healthy controls by nitrate reductase assay and diazo colorimetry, respectively. The comparative analysis of detected results was performed according to the grades of patients' liver function injury and LC with or without complications of cirrhosis.Results:The average concentrations of serum NO and NOS in LC patients were 127.67μmol/L and 4.25 U/ml,respectively, and significantly higher (P<0.001) than those in normal controls.According to the Child- Pugh grades of patients' liver function, the levels of serum NO and NOS were higher in the Child C than those in the Child B (P<0.05) or in Child A (P<0.01). The levels of serum NO and NOS were higher (P<0.05) in LC patients with the hemorrhage of esophageal varices, primary peritonitis and hypoxemia than those in LC patients without complication.Conclusions:The present data suggested that the overexpression of serum NO and NOS is closely associated with the LC severity and its complications.