人胎肝细胞和胎牛肝细胞低分子抑瘤物对小鼠腹腔积液型S-180细胞抑制作用的比较

目的：对比观察人胎肝细胞和胎牛肝细胞低分子天然抑瘤物（LMW－NTS）对小鼠腹腔积液型S－180细胞生长的抑制效应。方法：分别从胎儿肝细胞和胎牛肝细胞中分离提取出LMW－NTS。采用体外双层软琼脂培养法检测它们对S－180细胞集落生成的抑制作用；给两组荷糖小鼠每日分别腹腔注射人胎肝细胞和胎牛肝细胞LMW－NTS0．5ng/只，每日1次连连结注射21天，观察小鼠的寿命。结果：人胎肝细胞和胎牛肝细胞LMW－NTS对S－180细胞的生长均有明显的抑制作用，且抑制作用随LMW－NTS浓度增高而增强，腹腔注射治疗荷瘤小鼠均能显著延长小鼠帮助。结论：人胎肝细胞和胎牛肝细胞中均存在着一种作用相似的LMW－NTS，在体外均具有明显的抗肿瘤活性。

COMPARISON OF SUPPRESSIVE EFFECT BETWEEN THE LOW MOLECULAR WEIGHT NATURAL TUMOR SUPPRESSOR OF HUMAN FETAL HEPATOCYTE AND THAT OF EMBRYO CALF HEPATOCYTE ON THE GROWTH OF MURINE S-180 CELLS

Objective:Effect of the low molecular weight natural tumor suppressor (LMW NTS) of human fetal hepatocyte and that of embryo calf hepatocyte on the growth of murine S 180 cells was observed.Methods:LMW NTS was isolated respectively from human fetal hepatocyte and embryo calf hepatocyte.(1)In vitro two layers agar plate culture method was used to detect the suppressive effect of the human fetal hepatocyte LMW NTS and the emtryo calf hepatocyte LMW NTS on the growth of murine S 180 cells.(2)Surviral of the mice bearing S 180 cells and treated respectively with intraperitoneal injections of the human fetal hepatocyte LMW NTS or the embryo calf hepatocyte LMW NTS(0.5 mg/one mouse/day for 21 days)was surveyed.Results:Both the human fetal hepatocyte LMW NTS and the embryo calf hepatocyte LMW NTS can obviously inhibit the growth of murine S 180 cell,and the suppressive effect was enhanced while the concentration of LMW NTS was increased.Survival of the mice bearing tumor that treated with intraperitoneal injections of the human fetal hepatocyte LMW NTS or the embryo calf hepatocyte LMW NTS was remarkably prolonged.Conclusion:The LMW NTS of embryo calf hepatocyte is similar to that of human fetal hepatocyte,and it possesses marked anti tumor activity both in vitro and in vivo.