Immunological reaction between the peptides from S1 domain of SARS coronavirus S-protein and the serum from SARS patients] |
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| Authors: | Jia-jie Zhang Shao-yu Wu Wei Xu Jin-lin Hou Zhan-hui Wang Shi-bo Jiang Shu-guang Wu |
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| Institution: | Institute of Pharmaceutics Science, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China. jydyys@fimmu.com |
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| Abstract: | OBJECTIVE: To examine the immunological reactions between the peptides of SARS coronavirus (SARS-Cov) S-protein and the serum of SARS patients for identifying the SARS-Cov epitope. METHODS: The peptides from S1 domain of SARS-Cov S-protein were synthesized by peptide synthesizer, and the immunological reaction of the peptides with the serum of SARS patients were examined by means of ELISA. RESULTS: The optical density value of the immunological reaction of synthesized 8 peptides with SARS patient serum was 1.5-2.6 times higher than that with normal serum. CONCLUSION: The 8 peptides from S1 domain of S protein appear to have low immunogenicity to the serum of SARS patients, indicating that these peptides may not be the epitope of the SARS-Cov. |
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