Tumor necrosis factor-α alters protein metabolism and cell-cycle kinetics in malignant tumor |
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Authors: | Ye Shenglong Tang Zhaoyou Bruce R Bistrian |
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Institution: | (1) Liver Cancer Institute, Shanghai Medical University, 200032 Shanghai;(2) Cancer Research Institute, Deaconess Hospital, Harvard Medical School, Boston, USA |
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Abstract: | The effects of tumor necrosis factor-α (TNF) on protein metabolism and cell-cycle kinetics were investigated in malignant
tumor. Sprague-Dawley rats, subcutaneously inoculated with Walker 256 carcinosarcoma, were injected intraperitoneally with
recombinant human TNF at a dose of 4.75×106 U/kg for 3 consecutive days. Tumor protein metabolism and cell-cycle kinetics were analyzed. The results showed a significant
decrease in tumor volume and weight in comparison with control. TNF resulted in significant decrease in tumor protein fractional
synthesis rate, protein synthesis and fractional growth rate, but no change of tumor protein fractional degradation rate.
TNF also resulted in remarkable decline in labelling index and G1 phase increase of tumor cells, 6 hours after bromodeoxyuridine
injection, by cytometry. The results indicated that TNF inhibits tumor growth as a result of decreases in tumor cell DNA and
protein syntheses. |
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Keywords: | Tumor necrosis factor (TNF) Protein metabolism Cell-cycle Tumor experimental |
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